ALDOC - Antibodies - The Human Protein Atlas

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ALDOC

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA003282

Antibody HPA067442

Antibody CAB020828

Provider

Atlas Antibodies
Sigma-Aldrich

Origene

Product name

HPA003282

HPA067442

3291.00.02

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA003282, RRID:AB_1078130

VALIDATION SUMMARY

Antibody HPA003282

Antibody HPA067442

Antibody CAB020828

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA003282

Antibody HPA067442

Antibody CAB020828

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli fibrillar center & cytosol.

Antibody dilution

1:25

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA003282

Antibody HPA067442

Antibody CAB020828

Standard validation

Supported

Figure description

Immunohistochemical staining of human hippocampus shows strong positivity in subsets of glial cells.

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.

Expression

RNA: detected in 35 tissues
Protein: detected in 30 cell types

RNA: detected in 35 tissues
Protein: detected in 10 cell types

Retrieval

HIER pH6

Antibody dilution

1:400

1:2000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Standard validation

Supported

Figure description

Staining of Bergman glia in cerebellum and asterocytes in cerecral cortex.

WESTERN BLOT

Antibody HPA003282

Antibody HPA067442

Antibody CAB020828

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

39.5, 36.3, 19.7, 19.1, 16.3, 13.3

39.5, 36.3

39.5, 36.3, 19.7, 19.1, 16.3, 15, 13.3

Antibody dilution

1:250

1:140

1:500

PROTEIN ARRAY

Antibody HPA003282

Antibody HPA067442

Antibody CAB020828

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:1650

ANTIGEN INFORMATION

Antibody HPA003282

Antibody HPA067442

Antibody CAB020828

Antigen

Recombinant protein fragment

Genetic immunization

Length (aa)

Antigen sequence

MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVE
NTEENRRLYRQVLFSADDRVKKCIGGVI

QASALNAWRGQRDNAGAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAAQS
LYIANHAY

Matching transcripts

ALDOC-001 - ENSP00000226253 [100%]
ALDOC-002 - ENSP00000378729 [100%]
ALDOC-003 - ENSP00000378731 [100%]
ALDOC-004 - ENSP00000398976 [100%]
ALDOC-005 - ENSP00000463174 [100%]
ALDOC-006 - ENSP00000462674 [100%]
ALDOC-007 - ENSP00000463118 [100%]

ALDOC-001 - ENSP00000226253 [100%]
ALDOC-002 - ENSP00000378729 [100%]
ALDOC-003 - ENSP00000378731 [100%]

Other gene match

ANTIGEN VIEW

ALDOC-001	ALDOC-002	ALDOC-003	ALDOC-004	ALDOC-005	ALDOC-006	ALDOC-007

RELEVANT PUBLICATIONS

Antibody HPA003282

Antibody HPA067442

Antibody CAB020828

Bevacizumab treatment induces metabolic adaptation toward anaerobic metabolism in glioblastomas
Fack F et al
Acta Neuropathol 2015;129(1):115-31
Application: WB

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.