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CWH43

ANTIBODY INFORMATION
 
 

Antibody HPA042814

  

Antibody HPA048140

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042814   HPA048140  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA042814, RRID:AB_2678182   Atlas Antibodies Cat#HPA048140, RRID:AB_2680280  

VALIDATION SUMMARY
 
 

Antibody HPA042814

  

Antibody HPA048140

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042814

  

Antibody HPA048140

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 17 tissues
Protein: detected in 59 cell types		   RNA: detected in 17 tissues
Protein: detected in 65 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:850  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042814

  

Antibody HPA048140

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 78.6, 75.5   78.6, 75.5  

Antibody dilution

 1:250   1:370  

PROTEIN ARRAY
 
 

Antibody HPA042814

  

Antibody HPA048140

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:20000  

ANTIGEN INFORMATION
 
 

Antibody HPA042814

  

Antibody HPA048140

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 101   89  

Antigen sequence

 
GNVKDIDSTDHDRWCEYIMYRGLIRLGYARISHAELSDSEIQMAKFRIPD
DPTNYRDNQKVVIDHREVSEKIHFNPRFGSYKEGHNYENNHHFHMNTPKY
F
  
KAYERKLGKVAPTKEVSAAIWPFRFGYDNEGWSSLERSAHLLNETGADFI
TILESDASKPYMGNNDLTMWLGEKLGFYTDFGPSTRYHT
 

Matching transcripts

 CWH43-001 - ENSP00000226432 [100%]
CWH43-002 - ENSP00000422802 [100%]
  CWH43-001 - ENSP00000226432 [100%]
CWH43-002 - ENSP00000422802 [100%]
 

Other gene match

    
ANTIGEN VIEW
CWH43-001
CWH43-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.