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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Antibody dilution
1:29
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA000262
Antibody HPA006317
Antibody HPA006503
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear membrane positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 36 tissues Protein: detected in 39 cell types
RNA: detected in 36 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:200
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Supported
Figure description
Neuronal positivity observed in several brain regions, synaptic staining was also observed.
WESTERN BLOT
Antibody HPA000262
Antibody HPA006317
Antibody HPA006503
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
143.2
143.2
143.2
Antibody dilution
1:500
1:250
1:250
PROTEIN ARRAY
Antibody HPA000262
Antibody HPA006317
Antibody HPA006503
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
