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ANTIBODY INFORMATION
 
 

Antibody HPA038653

  

Antibody HPA038654

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA038653   HPA038654  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   7  

References

    

Proper citation

 Atlas Antibodies Cat#HPA038653, RRID:AB_10673016   Atlas Antibodies Cat#HPA038654, RRID:AB_10673285  

VALIDATION SUMMARY
 
 

Antibody HPA038653

  

Antibody HPA038654

  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038653

  

Antibody HPA038654

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in granular pattern in hepatocytes.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 42 cell types		   RNA: detected in 5 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:100  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038653

  

Antibody HPA038654

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39.5, 22.4   39.5, 31.5, 22.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA038653

  

Antibody HPA038654

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:12000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA038653

  

Antibody HPA038654

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   73  

Antigen sequence

 
AIPDPSWKDTVLGFRKLTPRELDMFPDYGYGWFHTSLILEGKNYLQWLTE
RLTERGVKFFQRKVESFEEVAREGADVIV
  
QPLDIKVYADRFTPLTTTDVAAGLWQPYLSDPNNPQEADWSQQTFDYLLS
HVHSPNAENLGLFLISGYNLFHE
 

Matching transcripts

 DAO-001 - ENSP00000228476 [100%]
DAO-009 - ENSP00000447104 [100%]
  DAO-001 - ENSP00000228476 [100%]
DAO-006 - ENSP00000446853 [100%]
DAO-009 - ENSP00000447104 [100%]
 

Other gene match

    
ANTIGEN VIEW
DAO-001
DAO-006
DAO-009
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.