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ATP5B

ANTIBODY INFORMATION
 
 

Antibody HPA001520

  

Antibody HPA001528

  

Antibody CAB017527

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA001520   HPA001528   sc-55597  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 2   2   4  

References

 3      

Proper citation

 Atlas Antibodies Cat#HPA001520, RRID:AB_1078243   Atlas Antibodies Cat#HPA001528, RRID:AB_1078242   Santa Cruz Biotechnology Cat#sc-55597, RRID:AB_1121153  

VALIDATION SUMMARY
 
 

Antibody HPA001520

  

Antibody HPA001528

  

Antibody CAB017527

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001520

  

Antibody HPA001528

  

Antibody CAB017527

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA001528.		  
Validated

Antibody staining overlaps with antibody HPA001520.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.    

Antibody dilution

 1:95   1:14    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001520

  

Antibody HPA001528

  

Antibody CAB017527

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA001528 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA001520 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human stomach shows strong positivity in granular pattern in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 57 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 51 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:50   1:800  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001520

  

Antibody HPA001528

  

Antibody CAB017527

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 56.6   56.6, 38.1   56.6, 38.1, 30.2, 28.4, 13.9  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001520

  

Antibody HPA001528

  

Antibody CAB017527

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1000    

ANTIGEN INFORMATION
 
 

Antibody HPA001520

  

Antibody HPA001528

  

Antibody CAB017527

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 142   139    

Antigen sequence

 
TSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEV
AQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIG
EPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVD
  
LTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQP
TLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATT
VLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVAR
  

											 
																				

																		

										
											
Matching transcripts

												
																					ATP5B-201 - ENSP00000262030 [100%] 

											 
																						ATP5B-004 - ENSP00000446489 [100%] 
ATP5B-201 - ENSP00000262030 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATP5B-004
ATP5B-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001520

												
										 
																				
											
											
											
Antibody HPA001528

												
										 
																				
											
											
											
Antibody CAB017527

												
										 
																		

																	

										
											
1.

												
																						Surrogate antigens as targets for proteome-wide binder selection

	Gustavsson E et al

	N Biotechnol 2011;28(4):302-11

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Resveratrol induces SIRT1- and energy-stress-independent inhibition of tumor cell regrowth after low-dose platinum treatment

	Björklund M et al

	Cancer Chemother Pharmacol 2011;68(6):1459-67

			
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization

	Desmurs M et al

	Mol Cell Biol 2015;35(7):1139-56

			Application: WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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