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KRT18

ANTIBODY INFORMATION
 
 

Antibody HPA001605

  

Antibody CAB000008

  

Antibody CAB000030

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Becton Dickinson
   SIGMA
  

Product name

 HPA001605   349205   C 1399  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Not known  

Other gene match

      

Released in version

 1   1   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA001605, RRID:AB_2666381      

VALIDATION SUMMARY
 
 

Antibody HPA001605

  

Antibody CAB000008

  

Antibody CAB000030

  
Immunocytochemistry

Supported      
Immunohistochemistry

Supported  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001605

  

Antibody CAB000008

  

Antibody CAB000030

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.      

Antibody dilution

 1:15      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001605

  

Antibody CAB000008

  

Antibody CAB000030

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.  
 

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human placenta shows distinct membranous and cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 50 cell types		   RNA: detected in 35 tissues
Protein: detected in 33 cell types		   RNA: detected in 35 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6   Proteinase K.   HIER pH9  

Antibody dilution

 1:50   1:10   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001605

  

Antibody CAB000008

  

Antibody CAB000030

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 48.1, 43.8   48.1, 43.8   48.1, 43.8  

Antibody dilution

 1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001605

  

Antibody CAB000008

  

Antibody CAB000030

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:1000      

ANTIGEN INFORMATION
 
 

Antibody HPA001605

  

Antibody CAB000008

  

Antibody CAB000030

  

Antigen

 Recombinant protein fragment   Not known   Not known  

Length (aa)

 113      

Antigen sequence

 
IEESTTVVTTQSTEVGAAKAMLTELTRTVQFLEINLDSMRNLKASLENSL
REVVARYALQMEQLNGILLHLESELAQTWAEGQCQAQEYQALLNIKVKLE
AAIATYRRLLEDG
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					KRT18-001 - ENSP00000447278 [89%] 
KRT18-002 - ENSP00000373487 [89%] 
KRT18-201 - ENSP00000373489 [89%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
KRT18-001
KRT18-002
KRT18-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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