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ACSS3

ANTIBODY INFORMATION
 
 

Antibody HPA039353

  

Antibody HPA047956

  

Antibody HPA061517

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039353   HPA047956   HPA061517  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 11   14   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA039353, RRID:AB_2676460   Atlas Antibodies Cat#HPA047956, RRID:AB_2680223   Atlas Antibodies Cat#HPA061517, RRID:AB_2684549  

VALIDATION SUMMARY
 
 

Antibody HPA039353

  

Antibody HPA047956

  

Antibody HPA061517

  
Immunocytochemistry

    Approved  
Immunohistochemistry

Approved   Approved    
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039353

  

Antibody HPA047956

  

Antibody HPA061517

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

     1:200  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039353

  

Antibody HPA047956

  

Antibody HPA061517

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunohistochemical staining of human liver shows STRONG CYTOPLASMIC positivity in hepatocytes.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.    

Expression

 RNA: detected in 31 tissues
Protein: detected in 65 cell types		   RNA: detected in 31 tissues
Protein: detected in 12 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:125   1:1000    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA039353

  

Antibody HPA047956

  

Antibody HPA061517

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 74.8, 74.6   74.8, 74.6   74.8, 74.6  

Antibody dilution

 1:250   1:450   1:890  

PROTEIN ARRAY
 
 

Antibody HPA039353

  

Antibody HPA047956

  

Antibody HPA061517

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3600   1:10600  

ANTIGEN INFORMATION
 
 

Antibody HPA039353

  

Antibody HPA047956

  

Antibody HPA061517

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   95   98  

Antigen sequence

 
EYKTHFAASVTDPERFWGKAAEQISWYKPWTKTLENKHSPSTRWFVEGML
NICYNAVDRHIENGKGDKIAIIYDSPVTNTKATFTYKEVLEQ
  
ASKELSSRIDHVKPKVVVTASFGIEPGRRVEYVPLVEEALKIGQHKPDKI
LIYNRPNMEAVPLAPGRDLDWDEEMAKAQSHDCVPVLSEHPLYIL
  
MDEEGYLYVMSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDP
LKGHVPLALCVLRKDINATEEQVLEEIVKHVRQNIGPVAAFRNAVFVK
 

Matching transcripts

 ACSS3-001 - ENSP00000449535 [100%]
ACSS3-002 - ENSP00000261206 [99%]
  ACSS3-001 - ENSP00000449535 [100%]
ACSS3-002 - ENSP00000261206 [100%]
  ACSS3-001 - ENSP00000449535 [100%]
ACSS3-002 - ENSP00000261206 [100%]
 

Other gene match

      
ANTIGEN VIEW
ACSS3-001
ACSS3-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.