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ACAD10

ANTIBODY INFORMATION
 
 

Antibody HPA067222

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA067222  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 14  

References

  

Proper citation

 Atlas Antibodies Cat#HPA067222, RRID:AB_2685805  

VALIDATION SUMMARY
 
 

Antibody HPA067222

  
Immunohistochemistry

Uncertain  
Western blot

Supported  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA067222

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in cells in non-germinal center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 33 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:2000  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA067222

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 122.3, 118.8, 80.5, 11.1  

Antibody dilution

 1:370  

PROTEIN ARRAY
 
 

Antibody HPA067222

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4400  

ANTIGEN INFORMATION
 
 

Antibody HPA067222

  

Antigen

 Recombinant protein fragment  

Length (aa)

 85  

Antigen sequence

 
MCVRSCFQSPRLQWVWRTAFLKHTQRRHQGSHRWTHLGGSTYRAVIFDMG
GVLIPSPGRVAAEWEVQNRIPSGTILKALMEGGEN
 

Matching transcripts

 ACAD10-001 - ENSP00000325137 [100%]
ACAD10-002 - ENSP00000389813 [100%]
ACAD10-009 - ENSP00000421527 [100%]
ACAD10-018 - ENSP00000446959 [100%]
 

Other gene match

  
ANTIGEN VIEW
ACAD10-001
ACAD10-002
ACAD10-009
ACAD10-018
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.