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ANTIBODY INFORMATION
 
 

Antibody HPA012388

  

Antibody HPA067448

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA012388   HPA067448  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA012388, RRID:AB_1854860   Atlas Antibodies Cat#HPA067448, RRID:AB_2685843  

VALIDATION SUMMARY
 
 

Antibody HPA012388

  

Antibody HPA067448

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012388

  

Antibody HPA067448

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA067448 across 70 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA012388 across 70 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.  

Expression

 RNA: detected in 11 tissues
Protein: detected in 23 cell types		   RNA: detected in 11 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:300  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012388

  

Antibody HPA067448

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 46.9, 42.1, 39.6   46.9  

Antibody dilution

 1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA012388

  

Antibody HPA067448

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2150  

ANTIGEN INFORMATION
 
 

Antibody HPA012388

  

Antibody HPA067448

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 138   92  

Antigen sequence

 
CCKDFESLCSDHEVSHSSDAITKEEIQSISEKIYRADTNKAQKEDIVLNS
QNCISPSETRNQVDRCPKPLFTYVNEKLFSKPTYAAFINLLNNYQRATGH
GEHFSAQELAEQDAFLREIMKTAVMKELYSFLHHQNRY
  
KIESCASRCNEKFNRDAACQCDRRCLWHGNCCEDYEHLCTEDHKESEPLP
QLEEETEEALASNLYSAPTSCQGRCYEAFDKHHQCHCNARCQ
 

Matching transcripts

 ENDOU-001 - ENSP00000229003 [100%]
ENDOU-002 - ENSP00000397679 [100%]
ENDOU-003 - ENSP00000445004 [100%]
  ENDOU-002 - ENSP00000397679 [100%]
 

Other gene match

    
ANTIGEN VIEW
ENDOU-001
ENDOU-002
ENDOU-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.