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CHD4

ANTIBODY INFORMATION
 
 

Antibody HPA012008

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA012008  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

  

Proper citation

 Atlas Antibodies Cat#HPA012008, RRID:AB_1846633  

VALIDATION SUMMARY
 
 

Antibody HPA012008

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Supported  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA012008

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		  

 

Figure description

 Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:24  
 

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:20  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012008

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:30  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012008

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 220.5, 218, 217.1, 39.1, 13.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA012008

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA012008

  

Antigen

 Recombinant protein fragment  

Length (aa)

 121  

Antigen sequence

 
DALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKR
QKKELGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKK
SKSKRKEEEEEDDDDDDSKEP
 

Matching transcripts

 CHD4-002 - ENSP00000440542 [99%]
CHD4-003 - ENSP00000437506 [94%]
CHD4-201 - ENSP00000349508 [94%]
CHD4-013 - ENSP00000440392 [91%]
CHD4-004 - ENSP00000444614 [87%]
 

Other gene match

  
ANTIGEN VIEW
CHD4-002
CHD4-003
CHD4-004
CHD4-013
CHD4-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.