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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Antibody dilution
1:53
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA004943
Antibody HPA050892
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human liver shows strong membranous positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate membranous positivity in hepatocytes.
Expression
RNA: detected in 3 tissues Protein: detected in 2 cell types
RNA: detected in 3 tissues Protein: detected in 1 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:200
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004943
Antibody HPA050892
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412714)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
77.4
82.5, 77.4, 76.4, 76.3, 71.2, 52.8
Antibody dilution
1:250
1:210
PROTEIN ARRAY
Antibody HPA004943
Antibody HPA050892
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
