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BTN3A3

ANTIBODY INFORMATION
 
 

Antibody HPA007904

  

Antibody HPA011871

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA007904   HPA011871  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   4  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA007904, RRID:AB_1078307   Atlas Antibodies Cat#HPA011871, RRID:AB_1845496  

VALIDATION SUMMARY
 
 

Antibody HPA007904

  

Antibody HPA011871

  
Immunohistochemistry


Validated
   Supported  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007904

  

Antibody HPA011871

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 66 cell types		   RNA: detected in 36 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:450   1:40  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007904

  

Antibody HPA011871

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 65, 59.7   65, 59.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA007904

  

Antibody HPA011871

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:12000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA007904

  

Antibody HPA011871

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 103   85  

Antigen sequence

 
SHIYTFPHASFSEPLYPVFRILTLEPTALTICPIPKEVESSPDPDLVPDH
SLETPLTPGLANESGEPQAEVTSLLLPAHPGAEVSPSATTNQNHKLQART
EAL
  
IALSRETEREREMKEMGYAATEQEISLREKLQEELKWRKIQYMARGEKSL
AYHEWKMALFKPADVILDPDTANAILLVSEDQRSV
 

Matching transcripts

 BTN3A3-001 - ENSP00000244519 [100%]
BTN3A3-002 - ENSP00000355238 [100%]
  BTN3A3-001 - ENSP00000244519 [100%]
BTN3A3-002 - ENSP00000355238 [92%]
 

Other gene match

    
ANTIGEN VIEW
BTN3A3-001
BTN3A3-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA007904

  

Antibody HPA011871

  

1.

 BTN3A2 expression in epithelial ovarian cancer is associated with higher tumor infiltrating T cells and a better prognosis
Le Page C et al
PLoS One 2012;7(6):e38541
    

2.

 The National NeuroAIDS Tissue Consortium brain gene array: two types of HIV-associated neurocognitive impairment
Gelman BB et al
PLoS One 2012;7(9):e46178
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.