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Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:29
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035738
Antibody HPA035739
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nucleolar positivity in neuronal cells.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:20
1:400
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035738
Antibody HPA035739
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402801)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402801)
Target mass (kDa)
50.5
50.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA035738
Antibody HPA035739
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
ANTIGEN INFORMATION
Antibody HPA035738
Antibody HPA035739
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
32
51
Antigen sequence
TNEPETNCNPSLLSIIGYNTTSTVPKEGQSVQ
RTSNNSQVNKLTLTSDESTLIEDGGARSDGSLEDGDDVHRAVDNERDGVT
Y
