Fields »
ABOUT
HELP
BLOG
NCOA7

ANTIBODY INFORMATION
 
 

Antibody HPA030289

  

Antibody HPA030290

  

Antibody HPA030291

  

Antibody HPA030292

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030289   HPA030290   HPA030291   HPA030292  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

        

Released in version

 7   6   7   7  

References

   1      

Proper citation

 Atlas Antibodies Cat#HPA030289, RRID:AB_10670031   Atlas Antibodies Cat#HPA030290, RRID:AB_10601971   Atlas Antibodies Cat#HPA030291, RRID:AB_10669597   Atlas Antibodies Cat#HPA030292, RRID:AB_10670834  

VALIDATION SUMMARY
 
 

Antibody HPA030289

  

Antibody HPA030290

  

Antibody HPA030291

  

Antibody HPA030292

  
Immunocytochemistry

      Approved  
Immunohistochemistry

Approved   Approved   Approved   Approved  
Western blot

Uncertain   Supported   Uncertain   Supported  
Protein array

Supported   Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030289

  

Antibody HPA030290

  

Antibody HPA030291

  

Antibody HPA030292

  

Standard validation

       Approved  

       

Figure description

       Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.  

Antibody dilution

       1:43  

Literature conformity

       The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030289

  

Antibody HPA030290

  

Antibody HPA030291

  

Antibody HPA030292

  

Standard validation

 Approved   Approved   Approved   Approved  

       

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cervix, uterine shows strong nuclear and additional cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 49 cell types		   RNA: detected in 37 tissues
Protein: detected in 34 cell types		   RNA: detected in 37 tissues
Protein: detected in 48 cell types		   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:90   1:225   1:350   1:70  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030289

  

Antibody HPA030290

  

Antibody HPA030291

  

Antibody HPA030292

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 106.2, 92.5   106.2, 92.5   106.2, 92.5   106.2, 92.5  

Antibody dilution

 1:250   1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA030289

  

Antibody HPA030290

  

Antibody HPA030291

  

Antibody HPA030292

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA030289

  

Antibody HPA030290

  

Antibody HPA030291

  

Antibody HPA030292

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 75   64   84   100  

Antigen sequence

 
NDVELKGALDLETCEKQDIMPEVDKQSGSPESRVENTLNIHEDLDKVKLI
EYYLTKNKEGPQVSENLQKTELSDG
  
EEVVSIALYNDISHMKIKDALPSPGEWEDLASEKDINPFSKFKSINKEKR
QQNGEKIMTSDSRP
  
HGSPTVTKLSKEPSDTSAAFESTAKENFLGEDDDFVDLEELSSQTGGGMH
KKDTLKECLSLDPEERKKAESQINNSAVEMQVQS
  
EPLPVKLNSSTEANVIKEALDSSLESTLDNSCQGAQMDNKSEVQLWLLKR
IQVPIEDILPSKEEKSKTPPMFLCIKVGKPMRKSFATHTAAMVQQYGKRR
 

Matching transcripts

 NCOA7-005 - ENSP00000357341 [100%]
NCOA7-012 - ENSP00000229634 [100%]
NCOA7-201 - ENSP00000376269 [100%]
  NCOA7-012 - ENSP00000229634 [100%]
NCOA7-005 - ENSP00000357341 [85%]
NCOA7-201 - ENSP00000376269 [85%]
  NCOA7-005 - ENSP00000357341 [99%]
NCOA7-012 - ENSP00000229634 [99%]
NCOA7-201 - ENSP00000376269 [99%]
  NCOA7-005 - ENSP00000357341 [100%]
NCOA7-012 - ENSP00000229634 [100%]
NCOA7-201 - ENSP00000376269 [100%]
 

Other gene match

        
ANTIGEN VIEW
NCOA7-005
NCOA7-012
NCOA7-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA030289

  

Antibody HPA030290

  

Antibody HPA030291

  

Antibody HPA030292

  

1.

   Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.