MAPK14 - Antibodies - The Human Protein Atlas

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MAPK14

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA051825

Antibody CAB010285

Antibody CAB040578

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

NCI-CPTC

Product name

HPA051825

sc-728

CPTC-MAPK14-1

Host species

Rabbit

Mouse

Clonality

pAb

msAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Protein A/G

Other gene match

Released in version

References

Proper citation

Santa Cruz Biotechnology Cat#sc-728, RRID:AB_632140

VALIDATION SUMMARY

Antibody HPA051825

Antibody CAB010285

Antibody CAB040578

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA051825

Antibody CAB010285

Antibody CAB040578

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.

Antibody dilution

1:10

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA051825

Antibody CAB010285

Antibody CAB040578

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows moderate positivity in tubules.

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 45 cell types

RNA: detected in 37 tissues
Protein: detected in 58 cell types

Retrieval

HIER pH6

Antibody dilution

1:500

1:30000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA051825

Antibody CAB010285

Antibody CAB040578

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

41.5, 41.3, 32.4

41.5, 41.3, 35.5, 32.4, 19.9, 15.5

Antibody dilution

1:100

1:500

1:250

PROTEIN ARRAY

Antibody HPA051825

Antibody CAB010285

Antibody CAB040578

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA051825

Antibody CAB010285

Antibody CAB040578

Antigen

Recombinant protein fragment

Synthetic peptide

Recombinant protein

Length (aa)

Antigen sequence

HDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES

Matching transcripts

MAPK14-001 - ENSP00000229795 [100%]
MAPK14-002 - ENSP00000229794 [100%]
MAPK14-003 - ENSP00000419837 [100%]

Other gene match

ANTIGEN VIEW

MAPK14-001	MAPK14-002	MAPK14-003

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.