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RNF8

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GENERAL INFORMATION

Gene name

 RNF8

Gene description

 Ring finger protein 8, E3 ubiquitin protein ligase

Protein class

 Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 4
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Mainly localized to the nucleus. In addition localized to the cytosol.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleus (supported)

Additional location

 In addition localized to the Cytosol (approved)

DATA RELIABILITY

Reliability score

 Supported based on 1 antibody.
HPA064925
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ASSAYS
RNA expression
Human cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Mainly localized to the nucleus. In addition localized to the cytosol.

Main location

 Nucleus (supported)

Additional location

 Cytosol (approved)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA064925 RH-30 Nucleus
Cytosol
HPA064925 U-2 OS Nucleus
Cytosol
HPA064925 U-251 MG No staining    

GENE INFORMATION

Gene name

 RNF8 (HGNC Symbol)

Synonyms

 KIAA0646

Description

 Ring finger protein 8, E3 ubiquitin protein ligase (HGNC Symbol)

Entrez gene summary

 The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Chromosome

 6

Cytoband

 p21.2

Chromosome location (bp)

 37353972 - 37394738

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000112130 (version 83.38)

Entrez gene

 9025

UniProt

 O76064 (UniProt - Evidence at protein level)

neXtProt

 NX_O76064

Antibodypedia

 RNF8 antibodies

PROTEIN BROWSER
RNF8-001
RNF8-002
RNF8-003
RNF8-007

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
RNF8-001
ENSP00000362578
ENST00000373479
O76064 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000151 [ubiquitin ligase complex]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0003682 [chromatin binding]
GO:0004842 [ubiquitin-protein transferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006511 [ubiquitin-dependent protein catabolic process]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007067 [mitotic nuclear division]
GO:0007286 [spermatid development]
GO:0008270 [zinc ion binding]
GO:0010212 [response to ionizing radiation]
GO:0016567 [protein ubiquitination]
GO:0016874 [ligase activity]
GO:0030496 [midbody]
GO:0031625 [ubiquitin protein ligase binding]
GO:0033522 [histone H2A ubiquitination]
GO:0033523 [histone H2B ubiquitination]
GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter]
GO:0035861 [site of double-strand break]
GO:0036297 [interstrand cross-link repair]
GO:0042393 [histone binding]
GO:0042803 [protein homodimerization activity]
GO:0043486 [histone exchange]
GO:0045190 [isotype switching]
GO:0045739 [positive regulation of DNA repair]
GO:0051301 [cell division]
GO:0051865 [protein autoubiquitination]
GO:0070534 [protein K63-linked ubiquitination]
GO:0070535 [histone H2A K63-linked ubiquitination]
GO:0070936 [protein K48-linked ubiquitination]
Show all
 485 aa
55.5 kDa 		No 0
RNF8-002
ENSP00000417599
ENST00000498460
H7C4L7 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
 208 aa
23.7 kDa 		No 0
RNF8-003
ENSP00000418879
ENST00000469731
O76064 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000151 [ubiquitin ligase complex]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0003682 [chromatin binding]
GO:0004842 [ubiquitin-protein transferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006511 [ubiquitin-dependent protein catabolic process]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007067 [mitotic nuclear division]
GO:0007286 [spermatid development]
GO:0008270 [zinc ion binding]
GO:0010212 [response to ionizing radiation]
GO:0016874 [ligase activity]
GO:0030496 [midbody]
GO:0031625 [ubiquitin protein ligase binding]
GO:0033522 [histone H2A ubiquitination]
GO:0033523 [histone H2B ubiquitination]
GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter]
GO:0035861 [site of double-strand break]
GO:0036297 [interstrand cross-link repair]
GO:0042393 [histone binding]
GO:0042803 [protein homodimerization activity]
GO:0043486 [histone exchange]
GO:0045190 [isotype switching]
GO:0045739 [positive regulation of DNA repair]
GO:0051301 [cell division]
GO:0051865 [protein autoubiquitination]
GO:0070534 [protein K63-linked ubiquitination]
GO:0070535 [histone H2A K63-linked ubiquitination]
GO:0070936 [protein K48-linked ubiquitination]
Show all
 448 aa
50.8 kDa 		No 0
RNF8-007
ENSP00000417736
ENST00000487950
C9J858 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
 205 aa
23.1 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.