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RNF8

GENERAL INFORMATION

Gene name

 RNF8

Gene description

 Ring finger protein 8, E3 ubiquitin protein ligase

Protein class

 Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 4
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HUMAN PROTEIN ATLAS INFORMATION

RNA category
TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Mixed

Protein evidence

 Evidence at protein level

Protein expression
normal tissue

 Nucleolar expression in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

 Antibody staining mainly not consistent with RNA expression data.

Reliability score

 Approved based on 1 antibody.
HPA050731
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PROGNOSTIC SUMMARY
Prognostic marker in liver cancer (unfavourable), prostate cancer (unfavourable) and melanoma (unfavourable).
Liver cancer p<0.001
Prostate cancer p<0.001
Melanoma p<0.001
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RNA EXPRESSION OVERVIEW
TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY
HPA050731

GENE INFORMATION

Gene name

 RNF8 (HGNC Symbol)

Synonyms

 KIAA0646

Description

 Ring finger protein 8, E3 ubiquitin protein ligase (HGNC Symbol)

Entrez gene summary

 The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Chromosome

 6

Cytoband

 p21.2

Chromosome location (bp)

 37353972 - 37394738

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000112130 (version 83.38)

Entrez gene

 9025

UniProt

 O76064 (UniProt - Evidence at protein level)

neXtProt

 NX_O76064

Antibodypedia

 RNF8 antibodies

PROTEIN BROWSER
RNF8-001
RNF8-002
RNF8-003
RNF8-007

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
RNF8-001
ENSP00000362578
ENST00000373479
O76064 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
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Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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GO:0000151 [ubiquitin ligase complex]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0003682 [chromatin binding]
GO:0004842 [ubiquitin-protein transferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006511 [ubiquitin-dependent protein catabolic process]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007067 [mitotic nuclear division]
GO:0007286 [spermatid development]
GO:0008270 [zinc ion binding]
GO:0010212 [response to ionizing radiation]
GO:0016567 [protein ubiquitination]
GO:0016874 [ligase activity]
GO:0030496 [midbody]
GO:0031625 [ubiquitin protein ligase binding]
GO:0033522 [histone H2A ubiquitination]
GO:0033523 [histone H2B ubiquitination]
GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter]
GO:0035861 [site of double-strand break]
GO:0036297 [interstrand cross-link repair]
GO:0042393 [histone binding]
GO:0042803 [protein homodimerization activity]
GO:0043486 [histone exchange]
GO:0045190 [isotype switching]
GO:0045739 [positive regulation of DNA repair]
GO:0051301 [cell division]
GO:0051865 [protein autoubiquitination]
GO:0070534 [protein K63-linked ubiquitination]
GO:0070535 [histone H2A K63-linked ubiquitination]
GO:0070936 [protein K48-linked ubiquitination]
Show all
 485 aa
55.5 kDa 		No 0
RNF8-002
ENSP00000417599
ENST00000498460
H7C4L7 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
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 208 aa
23.7 kDa 		No 0
RNF8-003
ENSP00000418879
ENST00000469731
O76064 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
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Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000151 [ubiquitin ligase complex]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0003682 [chromatin binding]
GO:0004842 [ubiquitin-protein transferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006511 [ubiquitin-dependent protein catabolic process]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007067 [mitotic nuclear division]
GO:0007286 [spermatid development]
GO:0008270 [zinc ion binding]
GO:0010212 [response to ionizing radiation]
GO:0016874 [ligase activity]
GO:0030496 [midbody]
GO:0031625 [ubiquitin protein ligase binding]
GO:0033522 [histone H2A ubiquitination]
GO:0033523 [histone H2B ubiquitination]
GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter]
GO:0035861 [site of double-strand break]
GO:0036297 [interstrand cross-link repair]
GO:0042393 [histone binding]
GO:0042803 [protein homodimerization activity]
GO:0043486 [histone exchange]
GO:0045190 [isotype switching]
GO:0045739 [positive regulation of DNA repair]
GO:0051301 [cell division]
GO:0051865 [protein autoubiquitination]
GO:0070534 [protein K63-linked ubiquitination]
GO:0070535 [histone H2A K63-linked ubiquitination]
GO:0070936 [protein K48-linked ubiquitination]
Show all
 448 aa
50.8 kDa 		No 0
RNF8-007
ENSP00000417736
ENST00000487950
C9J858 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
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GO:0005515 [protein binding]
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 205 aa
23.1 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.