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Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

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Searches

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Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

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Score

Score

Score

Antibodies

Column

 
 
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RNF8
  • TISSUE

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    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

RNF8

Gene description

Ring finger protein 8, E3 ubiquitin protein ligase

Protein class

Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

4
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Mixed

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Nucleolar expression in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data.

Reliability score

Approved based on 1 antibody.
HPA050731
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in liver cancer (unfavourable), prostate cancer (unfavourable) and melanoma (unfavourable).
Liver cancer p<0.001
Prostate cancer p<0.001
Melanoma p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA050731

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Several cases of colorectal, prostate, testicular, breast, stomach, liver, pancreatic cancers along with few cases of melanomas, lymphomas, cervical, thyroid, lung and skin cancers displayed moderate to strong cytoplasmic and/or nucleolar staining. Remaining cancer tissues were weakly stained or negative.

GENE INFORMATION

Gene name

RNF8 (HGNC Symbol)

Synonyms

KIAA0646

Description

Ring finger protein 8, E3 ubiquitin protein ligase (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Chromosome

6

Cytoband

p21.2

Chromosome location (bp)

37353972 - 37394738

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000112130 (version 83.38)

Entrez gene

9025

UniProt

O76064 (UniProt - Evidence at protein level)

neXtProt

NX_O76064

Antibodypedia

RNF8 antibodies

PROTEIN BROWSER

RNF8-001
RNF8-002
RNF8-003
RNF8-007

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

RNF8-001
ENSP00000362578
ENST00000373479
O76064 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000151 [ubiquitin ligase complex]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0003682 [chromatin binding]
GO:0004842 [ubiquitin-protein transferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006511 [ubiquitin-dependent protein catabolic process]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007067 [mitotic nuclear division]
GO:0007286 [spermatid development]
GO:0008270 [zinc ion binding]
GO:0010212 [response to ionizing radiation]
GO:0016567 [protein ubiquitination]
GO:0016874 [ligase activity]
GO:0030496 [midbody]
GO:0031625 [ubiquitin protein ligase binding]
GO:0033522 [histone H2A ubiquitination]
GO:0033523 [histone H2B ubiquitination]
GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter]
GO:0035861 [site of double-strand break]
GO:0036297 [interstrand cross-link repair]
GO:0042393 [histone binding]
GO:0042803 [protein homodimerization activity]
GO:0043486 [histone exchange]
GO:0045190 [isotype switching]
GO:0045739 [positive regulation of DNA repair]
GO:0051301 [cell division]
GO:0051865 [protein autoubiquitination]
GO:0070534 [protein K63-linked ubiquitination]
GO:0070535 [histone H2A K63-linked ubiquitination]
GO:0070936 [protein K48-linked ubiquitination]
Show all
485 aa
55.5 kDa
No 0
RNF8-002
ENSP00000417599
ENST00000498460
H7C4L7 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
208 aa
23.7 kDa
No 0
RNF8-003
ENSP00000418879
ENST00000469731
O76064 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000151 [ubiquitin ligase complex]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0003682 [chromatin binding]
GO:0004842 [ubiquitin-protein transferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006511 [ubiquitin-dependent protein catabolic process]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007067 [mitotic nuclear division]
GO:0007286 [spermatid development]
GO:0008270 [zinc ion binding]
GO:0010212 [response to ionizing radiation]
GO:0016874 [ligase activity]
GO:0030496 [midbody]
GO:0031625 [ubiquitin protein ligase binding]
GO:0033522 [histone H2A ubiquitination]
GO:0033523 [histone H2B ubiquitination]
GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter]
GO:0035861 [site of double-strand break]
GO:0036297 [interstrand cross-link repair]
GO:0042393 [histone binding]
GO:0042803 [protein homodimerization activity]
GO:0043486 [histone exchange]
GO:0045190 [isotype switching]
GO:0045739 [positive regulation of DNA repair]
GO:0051301 [cell division]
GO:0051865 [protein autoubiquitination]
GO:0070534 [protein K63-linked ubiquitination]
GO:0070535 [histone H2A K63-linked ubiquitination]
GO:0070936 [protein K48-linked ubiquitination]
Show all
448 aa
50.8 kDa
No 0
RNF8-007
ENSP00000417736
ENST00000487950
C9J858 [Direct mapping]
E3 ubiquitin-protein ligase RNF8
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
205 aa
23.1 kDa
No 0

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