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ANTIBODY INFORMATION
 
 

Antibody HPA001113

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001113  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

 1  

Proper citation

 Atlas Antibodies Cat#HPA001113, RRID:AB_1851385  

VALIDATION SUMMARY
 
 

Antibody HPA001113

  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001113

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity with a granular pattern in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 30 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:150  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001113

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 71.4  

Antibody dilution

 1:500  

PROTEIN ARRAY
 
 

Antibody HPA001113

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA001113

  

Antigen

 Recombinant protein fragment  

Length (aa)

 119  

Antigen sequence

 
VGHPLGSTTQNLQDSEKPQKGILEKAGPPPYIKPVPPAQPPAKPHTRISS
RQHQASQPPLHLTYPYKAEVSRTDHPSHLQEDKPSPLLFPSLHNKHPQSK
ITAGLEHKNGEIKPKSRRR
 

Matching transcripts

 ICK-001 - ENSP00000263043 [100%]
ICK-201 - ENSP00000349458 [100%]
 

Other gene match

  
ANTIGEN VIEW
ICK-001
ICK-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA001113

  

1.

 Diagnostic biomarkers of prostate cancer
Häggarth L et al
Scand J Urol Nephrol 2011;45(1):60-7
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.