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Immunofluorescent staining of human cell line RH-30 shows localization to cell junctions.
Antibody dilution
1:90
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA014788
Antibody HPA018176
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human heart muscle shows strong membranous positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic and membranous positivity in myocytes.
Expression
RNA: detected in 26 tissues Protein: detected in 62 cell types
RNA: detected in 26 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014788
Antibody HPA018176
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
41.5
41.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA014788
Antibody HPA018176
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
