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Immunohistochemical staining of human lateral ventricle shows moderate positivity in neuropil.
Immunohistochemical staining of human lateral ventricle shows strong positivity in neuropil.
Expression
RNA: detected in 24 tissues Protein: detected in 13 cell types
RNA: detected in 24 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:70
1:1100
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047200
Antibody CAB045998
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
89.4, 88.4
89.4, 88.4, 19.3
Antibody dilution
1:130
1:500
PROTEIN ARRAY
Antibody HPA047200
Antibody CAB045998
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
