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ANTIBODY INFORMATION
 
 

Antibody HPA031217

  

Antibody HPA031219

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA031217   HPA031219  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   6  

References

    

Proper citation

 Atlas Antibodies Cat#HPA031217, RRID:AB_10599488   Atlas Antibodies Cat#HPA031219, RRID:AB_10601989  

VALIDATION SUMMARY
 
 

Antibody HPA031217

  

Antibody HPA031219

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031217

  

Antibody HPA031219

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & vesicles.  

Antibody dilution

 1:89   1:75  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031217

  

Antibody HPA031219

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA031219 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA031217 across 46 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong nucleolar and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human esophagus shows strong nucleolar and cytoplasmic positivity in squamous epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 66 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:225   1:175  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA031217

  

Antibody HPA031219

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 49.6   49.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA031217

  

Antibody HPA031219

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA031217

  

Antibody HPA031219

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 81   81  

Antigen sequence

 
LDALVFHFLGFRTEKRELPVLWHQCLLTLVQRYKADLATDQKEALLELLR
LQPHPQLSPEIRRELQSAVPRDVEDVPITVE
  
QQQEELEAEHGTGDKPAAPRERTTRLGPRMPQDGSDDEDEEWPTLEKAAT
MTAAGHHAEVVVDPEDERAIEMFMNKNPPAR
 

Matching transcripts

 BYSL-001 - ENSP00000230340 [100%]
  BYSL-001 - ENSP00000230340 [100%]
 

Other gene match

    
ANTIGEN VIEW
BYSL-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.