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IMPG1

ANTIBODY INFORMATION
 
 

Antibody HPA027142

  

Antibody HPA030332

  

Antibody HPA030333

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA027142   HPA030332   HPA030333  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 16   16   16  

References

      

Proper citation

 Atlas Antibodies Cat#HPA027142, RRID:AB_2672132   Atlas Antibodies Cat#HPA030332, RRID:AB_2673432   Atlas Antibodies Cat#HPA030333, RRID:AB_2673433  

VALIDATION SUMMARY
 
 

Antibody HPA027142

  

Antibody HPA030332

  

Antibody HPA030333

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027142

  

Antibody HPA030332

  

Antibody HPA030333

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells.   Immunohistochemical staining of human retina shows moderate to strong cytoplasmic positivity in photoreceptor cells.   Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 1 cell types		   RNA: detected in 5 tissues
Protein: detected in 2 cell types		   RNA: detected in 5 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:7000   1:800   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA027142

  

Antibody HPA030332

  

Antibody HPA030333

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

      

Target mass (kDa)

 89.4, 80.1, 17.7   89.4, 80.1   89.4, 80.1, 16.5  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA027142

  

Antibody HPA030332

  

Antibody HPA030333

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA027142

  

Antibody HPA030332

  

Antibody HPA030333

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 96   95   84  

Antigen sequence

 
NERTEEAECRCKPGYDSQGSLDGLEPGLCGPGTKECEVLQGKGAPCRLPD
HSENQAYKTSVKKFQNQQNNKVISKRNSELLTVEYEEFNHQDWEGN
  
DSNKIESEEVYHGTMEEDKQPEIYLTATDLKRLISKALEEEQSLDVGTIQ
FTDEIAGSLPAFGPDTQSELPTSFAVITEDATLSPELPPVEPQLE
  
DTGEYQDWVSICQQETFCLFDIGKNFSNSQEHLDLLQQRIKQRSFPDRKD
EISAEKTLGEPGETIVISTDVANVSLGPFPLTPD
 

Matching transcripts

 IMPG1-001 - ENSP00000358966 [100%]
IMPG1-002 - ENSP00000358968 [100%]
IMPG1-202 - ENSP00000481913 [100%]
  IMPG1-001 - ENSP00000358966 [100%]
IMPG1-202 - ENSP00000481913 [100%]
  IMPG1-001 - ENSP00000358966 [100%]
IMPG1-202 - ENSP00000481913 [100%]
IMPG1-201 - ENSP00000358980 [85%]
 

Other gene match

      
ANTIGEN VIEW
IMPG1-001
IMPG1-002
IMPG1-201
IMPG1-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.