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Immunofluorescent staining of human cell line U-2 OS shows localization to centrosome.
Antibody dilution
1:75
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA058235
Antibody HPA074879
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows strong nucleolar positivity in neuronal cells.
Expression
RNA: detected in 28 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
Antibody dilution
1:1200
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA058235
Antibody HPA074879
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
71.7
Antibody dilution
1:1350
PROTEIN ARRAY
Antibody HPA058235
Antibody HPA074879
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
