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NPHP3

ANTIBODY INFORMATION
 
 

Antibody HPA009150

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA009150  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

 1  

Proper citation

 Atlas Antibodies Cat#HPA009150, RRID:AB_1078086  

VALIDATION SUMMARY
 
 

Antibody HPA009150

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA009150

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human skin shows strong cytoplasmic positivity in epidermal cells.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:75  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA009150

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 150.9  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA009150

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA009150

  

Antigen

 Recombinant protein fragment  

Length (aa)

 105  

Antigen sequence

 
PEFAHSSIDVEGPFANVNRDDWDIAVASLLQVTPLFSHSLWSNTVRCYLI
YTDETQPEMDLFLKDYSPKLKRMCETMGYFFHAVYFPIDVENQYLTVRKW
EIEKS
 

Matching transcripts

 NPHP3-006 - ENSP00000338766 [100%]
 

Other gene match

  
ANTIGEN VIEW
NPHP3-006

RELEVANT PUBLICATIONS
 
 

Antibody HPA009150

  

1.

 A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome
Blandin G et al
Skelet Muscle 2013;3(1):3
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.