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CLCN2

ANTIBODY INFORMATION
 
 

Antibody HPA014545

  

Antibody HPA024108

  

Antibody CAB009397

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA014545   HPA024108   sc-20122  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 4   5   3  

References

      

Proper citation

 Atlas Antibodies Cat#HPA014545, RRID:AB_1846881   Atlas Antibodies Cat#HPA024108, RRID:AB_1846883   Santa Cruz Biotechnology Cat#sc-20122, RRID:AB_2079256  

VALIDATION SUMMARY
 
 

Antibody HPA014545

  

Antibody HPA024108

  

Antibody CAB009397

  
Immunocytochemistry

Approved      
Immunohistochemistry


Validated
   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA014545

  

Antibody HPA024108

  

Antibody CAB009397

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol.      

Antibody dilution

 1:50      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014545

  

Antibody HPA024108

  

Antibody CAB009397

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.      
 

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity with a granular pattern in glandular cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 61 cell types		   RNA: detected in 34 tissues
Protein: detected in 56 cell types		   RNA: detected in 34 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:15   1:45  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA014545

  

Antibody HPA024108

  

Antibody CAB009397

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

      

Target mass (kDa)

 98.5, 96.8, 93.6   98.5, 96.8, 95.4, 93.6   98.5, 96.8, 95.4, 93.6  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA014545

  

Antibody HPA024108

  

Antibody CAB009397

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA014545

  

Antibody HPA024108

  

Antibody CAB009397

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 70   86    

Antigen sequence

 
GVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATS
SSDTETTEVHALWGPHSRHG
  
VCFQVNTEDSAFPAARGETHKPLKPALKRGPSVTRNLGESPTGSAESAGI
ALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLAS
  

											 
																				

																		

										
											
Matching transcripts

												
																					CLCN2-001 - ENSP00000265593 [100%] 
CLCN2-003 - ENSP00000400425 [100%] 
CLCN2-004 - ENSP00000345056 [100%] 

											 
																						CLCN2-001 - ENSP00000265593 [100%] 
CLCN2-002 - ENSP00000391928 [100%] 
CLCN2-003 - ENSP00000400425 [100%] 
CLCN2-004 - ENSP00000345056 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CLCN2-001
CLCN2-002
CLCN2-003
CLCN2-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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