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EIF4G1

ANTIBODY INFORMATION
 
 

Antibody HPA028487

  

Antibody HPA043866

  

Antibody CAB014774

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   BioSource
  

Product name

 HPA028487   HPA043866   44-526G  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 6   12   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA028487, RRID:AB_10602219   Atlas Antibodies Cat#HPA043866, RRID:AB_2678705    

VALIDATION SUMMARY
 
 

Antibody HPA028487

  

Antibody HPA043866

  

Antibody CAB014774

  
Immunocytochemistry


Validated
   Approved    
Immunohistochemistry

Approved     Approved  
Western blot


Validated
   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028487

  

Antibody HPA043866

  

Antibody CAB014774

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		      

     

Figure description

 Cytoplasmic region of segmented cells in 10x-images      

Antibody dilution

 1:23      
 

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.    

Antibody dilution

 1:20   1:120    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028487

  

Antibody HPA043866

  

Antibody CAB014774

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.     Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		     RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:50     1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028487

  

Antibody HPA043866

  

Antibody CAB014774

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 150, 100, 75, 50, 37, 25, 20, 15, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 176.2, 175.6, 175.5, 171.6, 171.5, 166.7, 166.6, 158.6, 158.5, 154.9, 154.8, 153.2, 98.2, 93.4, 89.2, 82.5, 29.3      

Loading control

 Total protein image      

Antibody dilution

 1:100      
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 176.2, 175.6, 175.5, 171.6, 171.5, 166.7, 166.6, 158.6, 158.5, 154.9, 154.8, 153.2, 98.2, 93.4, 89.2, 82.5, 29.3   176.2, 175.6, 175.5, 171.6, 171.5, 166.7, 166.6, 158.6, 158.5, 154.9, 154.8, 153.2   176.2, 175.6, 175.5, 171.6, 171.5, 166.7, 166.6, 158.6, 158.5, 154.9, 154.8, 153.2, 98.2, 93.4, 89.2, 82.5, 29.3, 24.7, 17, 16.9, 14.9, 14.8, 13, 9.9, 9.2  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA028487

  

Antibody HPA043866

  

Antibody CAB014774

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA028487

  

Antibody HPA043866

  

Antibody CAB014774

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 100   104    

Antigen sequence

 
QAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIA
PTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASVAPPTIPSATPATA
  
FSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGD
RLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVK
REAA
  

											 
																				

																		

										
											
Matching transcripts

												
																					EIF4G1-001 - ENSP00000343450 [99%] 
EIF4G1-003 - ENSP00000376320 [99%] 
EIF4G1-005 - ENSP00000403269 [99%] 
EIF4G1-006 - ENSP00000413159 [99%] 
EIF4G1-007 - ENSP00000316879 [99%] 
EIF4G1-008 - ENSP00000391412 [99%] 
EIF4G1-009 - ENSP00000317600 [99%] 
EIF4G1-010 - ENSP00000391935 [99%] 
EIF4G1-012 - ENSP00000407682 [99%] 
EIF4G1-013 - ENSP00000416255 [99%] 
EIF4G1-014 - ENSP00000371767 [99%] 
EIF4G1-015 - ENSP00000398145 [99%] 
EIF4G1-016 - ENSP00000411826 [99%] 
EIF4G1-029 - ENSP00000399969 [99%] 
EIF4G1-038 - ENSP00000395974 [99%] 
EIF4G1-039 - ENSP00000399858 [99%] 
EIF4G1-041 - ENSP00000404754 [99%] 
EIF4G1-201 - ENSP00000323737 [99%] 
EIF4G1-202 - ENSP00000338020 [99%] 

											 
																						EIF4G1-001 - ENSP00000343450 [100%] 
EIF4G1-003 - ENSP00000376320 [100%] 
EIF4G1-007 - ENSP00000316879 [100%] 
EIF4G1-009 - ENSP00000317600 [100%] 
EIF4G1-010 - ENSP00000391935 [100%] 
EIF4G1-012 - ENSP00000407682 [100%] 
EIF4G1-013 - ENSP00000416255 [100%] 
EIF4G1-014 - ENSP00000371767 [100%] 
EIF4G1-016 - ENSP00000411826 [100%] 
EIF4G1-038 - ENSP00000395974 [100%] 
EIF4G1-039 - ENSP00000399858 [100%] 
EIF4G1-041 - ENSP00000404754 [100%] 
EIF4G1-202 - ENSP00000338020 [100%] 
EIF4G1-201 - ENSP00000323737 [89%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

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EIF4G1-001
EIF4G1-003
EIF4G1-005
EIF4G1-006
EIF4G1-007
EIF4G1-008
EIF4G1-009
EIF4G1-010
EIF4G1-012
EIF4G1-013
EIF4G1-014
EIF4G1-015
EIF4G1-016
EIF4G1-029
EIF4G1-038
EIF4G1-039
EIF4G1-041
EIF4G1-201
EIF4G1-202
								

							

						

							
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