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EEF1B2

ANTIBODY INFORMATION
 
 

Antibody HPA035029

  

Antibody CAB012477

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA035029   ab37957  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA035029, RRID:AB_2674422   Abcam Cat#ab37957, RRID:AB_732078  

VALIDATION SUMMARY
 
 

Antibody HPA035029

  

Antibody CAB012477

  
Immunocytochemistry

Approved    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035029

  

Antibody CAB012477

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center & cytosol.    

Antibody dilution

 1:9    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035029

  

Antibody CAB012477

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.   Immunohistochemical staining of human cerebellum shows distinct cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:4000  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035029

  

Antibody CAB012477

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 24.8, 13.4   24.8, 13.4  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA035029

  

Antibody CAB012477

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA035029

  

Antibody CAB012477

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 92    

Antigen sequence

 
FGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHA
LRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATD
  

											 
																				

																		

										
											
Matching transcripts

												
																					EEF1B2-001 - ENSP00000236957 [100%] 
EEF1B2-003 - ENSP00000376056 [100%] 
EEF1B2-004 - ENSP00000407730 [100%] 
EEF1B2-201 - ENSP00000376055 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
EEF1B2-001
EEF1B2-003
EEF1B2-004
EEF1B2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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