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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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ZAP70
  • premium

    TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

ZAP70

Gene description

Zeta-chain (TCR) associated protein kinase 70kDa

Protein class

Disease related genes
Enzymes
Plasma proteins
Potential drug targets
Predicted intracellular proteins
RAS pathway related proteins

Predicted localization

Intracellular

Number of transcripts

2
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Mixed
HPA (cell line):Cell line enriched (MOLT-4)
HPA (normal tissue):Tissue enhanced (appendix, lymph node, spleen)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Selective cytoplasmic expression in subsets of immune cells.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining consistent with RNA expression data. Presumed off target binding observed and disregarded.

Reliability score

Supported based on 2 antibodies.
HPA003134 , CAB002625
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable), endometrial cancer (favourable), head and neck cancer (favourable) and cervical cancer (favourable).
Renal cancer p<0.001
Endometrial cancer p<0.001
Head and neck cancer p<0.001
Cervical cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Mixed

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA003134
CAB002625

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer tissues were negative. A few lymphomas, breast cancers and skin cancers displayed distinct nuclear immunoreactivity. Lymphoid cells of tumor stroma were strongly positive.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong cytoplasmic staining with a granular pattern was observed in a few hepatocellular carcinomas and lymphomas. Remaining cancer tissues were in general negative.

GENE INFORMATION

Gene name

ZAP70 (HGNC Symbol)

Synonyms

SRK, STD, ZAP-70

Description

Zeta-chain (TCR) associated protein kinase 70kDa (HGNC Symbol)

Entrez gene summary

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Chromosome

2

Cytoband

q11.2

Chromosome location (bp)

97713560 - 97739862

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000115085 (version 83.38)

Entrez gene

7535

UniProt

P43403 (UniProt - Evidence at protein level)

neXtProt

NX_P43403

Antibodypedia

ZAP70 antibodies

PROTEIN BROWSER

ZAP70-001
ZAP70-008

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ZAP70-001
ENSP00000264972
ENST00000264972
P43403 [Direct mapping]
Tyrosine-protein kinase ZAP-70
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Enzymes
   ENZYME proteins
   Transferases
   Kinases
   Tyr protein kinases
   SCAMPI predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
RAS pathway related proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001772 [immunological synapse]
GO:0001784 [phosphotyrosine binding]
GO:0002250 [adaptive immune response]
GO:0004672 [protein kinase activity]
GO:0004713 [protein tyrosine kinase activity]
GO:0004715 [non-membrane spanning protein tyrosine kinase activity]
GO:0005102 [receptor binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0005911 [cell-cell junction]
GO:0006468 [protein phosphorylation]
GO:0006955 [immune response]
GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway]
GO:0016020 [membrane]
GO:0018108 [peptidyl-tyrosine phosphorylation]
GO:0030154 [cell differentiation]
GO:0030217 [T cell differentiation]
GO:0031234 [extrinsic component of cytoplasmic side of plasma membrane]
GO:0035556 [intracellular signal transduction]
GO:0038083 [peptidyl-tyrosine autophosphorylation]
GO:0042101 [T cell receptor complex]
GO:0042110 [T cell activation]
GO:0042113 [B cell activation]
GO:0043366 [beta selection]
GO:0045059 [positive thymic T cell selection]
GO:0045060 [negative thymic T cell selection]
GO:0045061 [thymic T cell selection]
GO:0045087 [innate immune response]
GO:0045121 [membrane raft]
GO:0045582 [positive regulation of T cell differentiation]
GO:0046638 [positive regulation of alpha-beta T cell differentiation]
GO:0046641 [positive regulation of alpha-beta T cell proliferation]
GO:0046777 [protein autophosphorylation]
GO:0050850 [positive regulation of calcium-mediated signaling]
GO:0050852 [T cell receptor signaling pathway]
GO:0070489 [T cell aggregation]
GO:0072678 [T cell migration]
Show all
619 aa
69.9 kDa
No 0
ZAP70-008
ENSP00000400475
ENST00000451498
P43403 [Direct mapping]
Tyrosine-protein kinase ZAP-70
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Enzymes
   ENZYME proteins
   Transferases
   Kinases
   Tyr protein kinases
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
RAS pathway related proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001772 [immunological synapse]
GO:0002250 [adaptive immune response]
GO:0004672 [protein kinase activity]
GO:0004713 [protein tyrosine kinase activity]
GO:0004715 [non-membrane spanning protein tyrosine kinase activity]
GO:0005102 [receptor binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006468 [protein phosphorylation]
GO:0006955 [immune response]
GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway]
GO:0018108 [peptidyl-tyrosine phosphorylation]
GO:0030154 [cell differentiation]
GO:0030217 [T cell differentiation]
GO:0031234 [extrinsic component of cytoplasmic side of plasma membrane]
GO:0035556 [intracellular signal transduction]
GO:0038083 [peptidyl-tyrosine autophosphorylation]
GO:0042101 [T cell receptor complex]
GO:0042110 [T cell activation]
GO:0042113 [B cell activation]
GO:0045059 [positive thymic T cell selection]
GO:0045087 [innate immune response]
GO:0045121 [membrane raft]
GO:0045582 [positive regulation of T cell differentiation]
GO:0046641 [positive regulation of alpha-beta T cell proliferation]
GO:0050852 [T cell receptor signaling pathway]
GO:0070489 [T cell aggregation]
GO:0072678 [T cell migration]
Show all
312 aa
35.6 kDa
No 0

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