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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Antibody dilution
1:4
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA029527
Antibody CAB033078
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 62 cell types
RNA: detected in 37 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:15
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029527
Antibody CAB033078
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415614)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
60.8, 59.8, 17.5
60.8, 59.8, 17.5, 8.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA029527
Antibody CAB033078
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
