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Immunofluorescent staining of human cell line SH-SY5Y shows localization to the Golgi apparatus & vesicles.
Antibody dilution
1:9
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA036760
Antibody HPA036761
Antibody CAB000040
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet of Langerhans.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Expression
RNA: detected in 20 tissues Protein: detected in 47 cell types
RNA: detected in 20 tissues Protein: detected in 31 cell types
RNA: detected in 20 tissues Protein: detected in 2 cell types
Retrieval
HIER pH6
HIER pH6
None
Antibody dilution
1:1000
1:800
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036760
Antibody HPA036761
Antibody CAB000040
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
20.9
20.9
20.9
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA036760
Antibody HPA036761
Antibody CAB000040
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
