CCDC88A - Antibodies - The Human Protein Atlas

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CCDC88A

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA038101

Antibody HPA038102

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA038101

HPA038102

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA038101, RRID:AB_10670654

Atlas Antibodies Cat#HPA038102, RRID:AB_2675833

VALIDATION SUMMARY

Antibody HPA038101

Antibody HPA038102

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA038101

Antibody HPA038102

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Immunohistochemical staining of human appendix shows leukocyte positivity in Germinal center.

Expression

RNA: detected in 37 tissues
Protein: detected in 11 cell types

RNA: detected in 37 tissues
Protein: detected in 13 cell types

Retrieval

HIER pH6

Antibody dilution

1:60

1:250

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA038101

Antibody HPA038102

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

216, 212.6, 136.6, 100.6, 41

216, 212.6, 207.8, 136.6, 100.6, 83.5

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA038101

Antibody HPA038102

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA038101

Antibody HPA038102

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

TPGDFYDRRTTKPEFLRPGPRKTEDTYFISSAGKPTPGTQGKIKLVKESS
LSRQSKDSNPYATLPRASSVISTAEGTT

VPDDISTGKRRKELGAMAFSTTAINFSTVNSSAGFRSKQLVNNKDTTSFE
DISPQGVSDDSSTGSRVHASRPAS

Matching transcripts

CCDC88A-001 - ENSP00000263630 [100%]
CCDC88A-002 - ENSP00000338728 [100%]
CCDC88A-003 - ENSP00000413401 [100%]
CCDC88A-005 - ENSP00000405080 [100%]
CCDC88A-006 - ENSP00000390012 [100%]
CCDC88A-202 - ENSP00000410608 [100%]

CCDC88A-001 - ENSP00000263630 [100%]
CCDC88A-002 - ENSP00000338728 [100%]
CCDC88A-005 - ENSP00000405080 [100%]
CCDC88A-006 - ENSP00000390012 [100%]
CCDC88A-008 - ENSP00000415267 [100%]
CCDC88A-201 - ENSP00000404431 [100%]
CCDC88A-202 - ENSP00000410608 [100%]

Other gene match

ANTIGEN VIEW

CCDC88A-001	CCDC88A-002	CCDC88A-003	CCDC88A-005	CCDC88A-006	CCDC88A-008	CCDC88A-201	CCDC88A-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.