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Antibody staining overlaps with GFP tagged protein
Validated
Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.
Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.
Antibody dilution
1:45
1:60
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:45
1:61
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA000931
Antibody HPA000982
Antibody CAB004049
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human lymph node shows cytoplasmic positivity in non-germinal center cells.
Immunohistochemical staining of human lymph node shows cytoplasmic positivity in non-germinal center cells.
Immunohistochemical staining of human lymph node shows cytoplasmic positivity in non-germinal center cells.
Expression
RNA: detected in 37 tissues Protein: detected in 42 cell types
RNA: detected in 37 tissues Protein: detected in 14 cell types
RNA: detected in 37 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:500
1:50
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000931
Antibody HPA000982
Antibody CAB004049
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
87.3, 83.4, 83
87.3
87.3, 83.4, 83, 23.1, 21.8, 18.1, 8.6
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA000931
Antibody HPA000982
Antibody CAB004049
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
