DARS - Antibodies - The Human Protein Atlas

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DARS

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA020451

Antibody HPA029804

Antibody HPA029805

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA020451

HPA029804

HPA029805

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA020451, RRID:AB_1847479

Atlas Antibodies Cat#HPA029804, RRID:AB_10599178

Atlas Antibodies Cat#HPA029805, RRID:AB_10601994

VALIDATION SUMMARY

Antibody HPA020451

Antibody HPA029804

Antibody HPA029805

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA020451

Antibody HPA029804

Antibody HPA029805

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:25

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Antibody dilution

1:20

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA020451

Antibody HPA029804

Antibody HPA029805

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA029805 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA029804 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA029805 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA029804 across 75 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human breast shows cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 71 cell types

RNA: detected in 37 tissues
Protein: detected in 75 cell types

RNA: detected in 37 tissues
Protein: detected in 63 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:75

1:15

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA020451

Antibody HPA029804

Antibody HPA029805

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

57.1

57.1, 21.6

57.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA020451

Antibody HPA029804

Antibody HPA029805

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA020451

Antibody HPA029804

Antibody HPA029805

Antigen

Recombinant protein fragment

Length (aa)

125

112

135

Antigen sequence

LHVQKIYVISLAEPRLPLQLDDAVRPEAEGEEEGRATVNQDTRLDNRVID
LRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGANVFT
VSYFKNNAYLAQSPQLYKQMCICAD

AEDSNTHRHLTEFVGLDIEMAFNYHYHEVMEEIADTMVQIFKGLQERFQT
EIQTVNKQFPCEPFKFLEPTLRLEYCEALAMLREAGVEMGDEDDLSTPNE
KLLGHLVKEKYD

MPSASASRKSQEKPREIMDAAEDYAKERYGISSMIQSQEKPDRVLVRVRD
LTIQKADEVVWVRARVHTSRAKGKQCFLVLRQQQFNVQALVAVGDHASKQ
MVKFAANINKESIVDVEGVVRKVNQKIGSCTQQDV

Matching transcripts

DARS-001 - ENSP00000264161 [100%]

DARS-001 - ENSP00000264161 [100%]
DARS-009 - ENSP00000387508 [87%]

DARS-001 - ENSP00000264161 [100%]

Other gene match

ANTIGEN VIEW

DARS-001	DARS-009

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.