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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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AAK1
  • TISSUE

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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

AAK1

Gene description

AP2 associated kinase 1

Protein class

Enzymes
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

6
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Tissue enriched (parathyroid gland)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membrane expression at variable levels in several different cell types.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data.

Reliability score

Approved based on 2 antibodies.
HPA017931 , HPA020289
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PROGNOSTIC SUMMARY

Prognostic marker in ovarian cancer (unfavourable).
Ovarian cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA017931
HPA020289

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A majority of urothelial and several prostate, renal and cervical cancers. Most remaining cancer tissues were weakly stained or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues generally displayed moderate cytoplasmic immunoreactivity. Thyroid cancers and a few gliomas showed strong staining. Several lymphomas, testicular, pancreatic, gastric and endometrial cancers were weakly stained or negative.

GENE INFORMATION

Gene name

AAK1 (HGNC Symbol)

Synonyms

DKFZp686K16132, KIAA1048

Description

AP2 associated kinase 1 (HGNC Symbol)

Entrez gene summary

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Chromosome

2

Cytoband

p13.3

Chromosome location (bp)

69457997 - 69674349

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000115977 (version 83.38)

Entrez gene

22848

UniProt

Q2M2I8 (UniProt - Evidence at protein level)

neXtProt

NX_Q2M2I8

Antibodypedia

AAK1 antibodies

PROTEIN BROWSER

AAK1-001
AAK1-002
AAK1-011
AAK1-012
AAK1-013
AAK1-014

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

AAK1-001
ENSP00000386456
ENST00000409085
Q2M2I8 [Direct mapping]
AP2-associated protein kinase 1
Show all
Enzymes
   ENZYME proteins
   Transferases
   Kinases
   MEMSAT-SVM predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005112 [Notch binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005905 [coated pit]
GO:0006468 [protein phosphorylation]
GO:0006897 [endocytosis]
GO:0019897 [extrinsic component of plasma membrane]
GO:0030136 [clathrin-coated vesicle]
GO:0031252 [cell leading edge]
GO:0032880 [regulation of protein localization]
GO:0035612 [AP-2 adaptor complex binding]
GO:0043195 [terminal bouton]
GO:0045747 [positive regulation of Notch signaling pathway]
GO:0046777 [protein autophosphorylation]
GO:0050821 [protein stabilization]
GO:2000369 [regulation of clathrin-mediated endocytosis]
Show all
961 aa
103.9 kDa
No 0
AAK1-002
ENSP00000385181
ENST00000406297
Q2M2I8 [Direct mapping]
AP2-associated protein kinase 1
Show all
Enzymes
   ENZYME proteins
   Transferases
   Kinases
   MEMSAT-SVM predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0004674 [protein serine/threonine kinase activity]
GO:0005112 [Notch binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005905 [coated pit]
GO:0006468 [protein phosphorylation]
GO:0006897 [endocytosis]
GO:0019897 [extrinsic component of plasma membrane]
GO:0030136 [clathrin-coated vesicle]
GO:0031252 [cell leading edge]
GO:0032880 [regulation of protein localization]
GO:0035612 [AP-2 adaptor complex binding]
GO:0043195 [terminal bouton]
GO:0045747 [positive regulation of Notch signaling pathway]
GO:0046777 [protein autophosphorylation]
GO:0050821 [protein stabilization]
GO:2000369 [regulation of clathrin-mediated endocytosis]
Show all
863 aa
93.6 kDa
No 0
AAK1-011
ENSP00000386342
ENST00000409068
E9PG46 [Direct mapping]
AP2-associated protein kinase 1
Show all
   MEMSAT-SVM predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0005524 [ATP binding]
GO:0006468 [protein phosphorylation]
Show all
674 aa
73.8 kDa
No 0
AAK1-012
ENSP00000485350
ENST00000606389
A0A096LP25 [Direct mapping]
AP2-associated protein kinase 1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
511 aa
54.4 kDa
No 0
AAK1-013
ENSP00000485296
ENST00000623317
A0A096LNZ0 [Direct mapping]
AP2-associated protein kinase 1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
340 aa
36.4 kDa
No 0
AAK1-014
ENSP00000485409
ENST00000624900
A0A096LP60 [Direct mapping]
AP2-associated protein kinase 1
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
99 aa
10.3 kDa
No 0

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