Expression of AAK1 in cancer - Summary - The Human Protein Atlas

GENERAL INFORMATION
Gene name	AAK1
Gene description	AP2 associated kinase 1
Protein class	Enzymes Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	6
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Expressed in all
HPA (normal tissue):	Tissue enriched (parathyroid gland)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membrane expression at variable levels in several different cell types.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly not consistent with RNA expression data.
Reliability score	Approved based on 2 antibodies. HPA017931 , HPA020289
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PROGNOSTIC SUMMARY
Prognostic marker in ovarian cancer (unfavourable).
Ovarian cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

HPA017931	HPA020289

GENE INFORMATION
Gene name	AAK1 (HGNC Symbol)
Synonyms	DKFZp686K16132, KIAA1048
Description	AP2 associated kinase 1 (HGNC Symbol)
Entrez gene summary	Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Chromosome	2
Cytoband	p13.3
Chromosome location (bp)	69457997 - 69674349
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000115977 (version 83.38)
Entrez gene	22848
UniProt	Q2M2I8 (UniProt - Evidence at protein level)
neXtProt	NX_Q2M2I8
Antibodypedia	AAK1 antibodies

PROTEIN BROWSER

AAK1-001	AAK1-002	AAK1-011	AAK1-012	AAK1-013	AAK1-014

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
AAK1-001 ENSP00000386456 ENST00000409085	Q2M2I8 [Direct mapping] AP2-associated protein kinase 1 Show all	Enzymes ENZYME proteins Transferases Kinases MEMSAT-SVM predicted membrane proteins SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0004672 [protein kinase activity] GO:0004674 [protein serine/threonine kinase activity] GO:0005112 [Notch binding] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005905 [coated pit] GO:0006468 [protein phosphorylation] GO:0006897 [endocytosis] GO:0019897 [extrinsic component of plasma membrane] GO:0030136 [clathrin-coated vesicle] GO:0031252 [cell leading edge] GO:0032880 [regulation of protein localization] GO:0035612 [AP-2 adaptor complex binding] GO:0043195 [terminal bouton] GO:0045747 [positive regulation of Notch signaling pathway] GO:0046777 [protein autophosphorylation] GO:0050821 [protein stabilization] GO:2000369 [regulation of clathrin-mediated endocytosis] Show all	961 aa 103.9 kDa	No	0
AAK1-002 ENSP00000385181 ENST00000406297	Q2M2I8 [Direct mapping] AP2-associated protein kinase 1 Show all	Enzymes ENZYME proteins Transferases Kinases MEMSAT-SVM predicted membrane proteins SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0004672 [protein kinase activity] GO:0004674 [protein serine/threonine kinase activity] GO:0005112 [Notch binding] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005905 [coated pit] GO:0006468 [protein phosphorylation] GO:0006897 [endocytosis] GO:0019897 [extrinsic component of plasma membrane] GO:0030136 [clathrin-coated vesicle] GO:0031252 [cell leading edge] GO:0032880 [regulation of protein localization] GO:0035612 [AP-2 adaptor complex binding] GO:0043195 [terminal bouton] GO:0045747 [positive regulation of Notch signaling pathway] GO:0046777 [protein autophosphorylation] GO:0050821 [protein stabilization] GO:2000369 [regulation of clathrin-mediated endocytosis] Show all	863 aa 93.6 kDa	No	0
AAK1-011 ENSP00000386342 ENST00000409068	E9PG46 [Direct mapping] AP2-associated protein kinase 1 Show all	MEMSAT-SVM predicted membrane proteins SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0004672 [protein kinase activity] GO:0005524 [ATP binding] GO:0006468 [protein phosphorylation] Show all	674 aa 73.8 kDa	No	0
AAK1-012 ENSP00000485350 ENST00000606389	A0A096LP25 [Direct mapping] AP2-associated protein kinase 1 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		511 aa 54.4 kDa	No	0
AAK1-013 ENSP00000485296 ENST00000623317	A0A096LNZ0 [Direct mapping] AP2-associated protein kinase 1 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		340 aa 36.4 kDa	No	0
AAK1-014 ENSP00000485409 ENST00000624900	A0A096LP60 [Direct mapping] AP2-associated protein kinase 1 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		99 aa 10.3 kDa	No	0