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Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:20
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA025767
Antibody HPA025826
Antibody HPA025951
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA025826 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA025951 across 46 cell lines. Pearson correlation with HPA025767 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA025826 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA025951 across 75 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA025826 across 75 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human small intestine shows moderate granular positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 64 cell types
RNA: detected in 37 tissues Protein: detected in 72 cell types
RNA: detected in 37 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:15
1:40
1:15
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA025767
Antibody HPA025826
Antibody HPA025951
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
48.1, 32.5
55, 48.1, 32.5, 29.5
55, 48.1
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA025767
Antibody HPA025826
Antibody HPA025951
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
