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HDAC1

ANTIBODY INFORMATION
 
 

Antibody HPA029693

  

Antibody CAB005017

  

Antibody CAB068191

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Rockland
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA029693   600-401-879   AMAb90781  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

      

Released in version

 6   3   13  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA029693, RRID:AB_10601659   Rockland Cat#600-401-879, RRID:AB_2248344   Atlas Antibodies Cat#AMAb90781, RRID:AB_2665664  

VALIDATION SUMMARY
 
 

Antibody HPA029693

  

Antibody CAB005017

  

Antibody CAB068191

  
Immunocytochemistry


Validated

Validated
   Supported    
Immunohistochemistry

Supported   Supported  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029693

  

Antibody CAB005017

  

Antibody CAB068191

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA and > 10% by the other.		      

     

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:69      
 

Formal validation: Tagged protein
Validated

Antibody staining overlaps with GFP tagged protein		      

     

Figure description

 Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus but excluded from the nucleoli and GFP expression in nucleus but excluded from the nucleoli.      

Antibody dilution

 1:69      
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.    

Antibody dilution

 1:50   1:50    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029693

  

Antibody CAB005017

  

Antibody CAB068191

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human duodenum shows nuclear positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human tonsil shows strong nuclear positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 60 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:200   1:1000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA029693

  

Antibody CAB005017

  

Antibody CAB068191

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 55.1   55.1, 24.5   55.1, 24.5  

Antibody dilution

 1:250   1:500   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA029693

  

Antibody CAB005017

  

Antibody CAB068191

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA029693

  

Antibody CAB005017

  

Antibody CAB068191

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Recombinant protein  

Length (aa)

 65      

Antigen sequence

 
RIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEE
EKTKEEKPEAKGVKE
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					HDAC1-001 - ENSP00000362649 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
HDAC1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA029693

												
										 
																				
											
											
											
Antibody CAB005017

												
										 
																				
											
											
											
Antibody CAB068191

												
										 
																		

																	

										
											
1.

												
																						Recruitment of Oct4 protein to UV-damaged chromatin in embryonic stem cells

	Bártová E et al

	PLoS One 2011;6(12):e27281

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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