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ANTIBODY INFORMATION
 
 

Antibody HPA002327

  

Antibody HPA006040

  

Antibody CAB022160

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA002327   HPA006040   07-002  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Serum  

Other gene match

      

Released in version

 2   4   5  

References

   1    

Proper citation

 Atlas Antibodies Cat#HPA002327, RRID:AB_1079550   Atlas Antibodies Cat#HPA006040, RRID:AB_1854906    

VALIDATION SUMMARY
 
 

Antibody HPA002327

  

Antibody HPA006040

  

Antibody CAB022160

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002327

  

Antibody HPA006040

  

Antibody CAB022160

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & cytosol.  

Antibody dilution

   1:34   1:2000  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002327

  

Antibody HPA006040

  

Antibody CAB022160

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.   Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in bone marrow poietic cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 18 cell types		   RNA: detected in 36 tissues
Protein: detected in 37 cell types		   RNA: detected in 36 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:35   1:3000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002327

  

Antibody HPA006040

  

Antibody CAB022160

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 59.8, 54.4, 50.3   59.8   59.8, 54.4, 50.3, 16.2, 6.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002327

  

Antibody HPA006040

  

Antibody CAB022160

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA002327

  

Antibody HPA006040

  

Antibody CAB022160

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 148   147    

Antigen sequence

 
QEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVH
YKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSE
DSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQ
  
MLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFACEVLYNIA
FMYAKKEEWKKAEEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIP
VGRLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAA
  

											 
																				

																		

										
											
Matching transcripts

												
																					NCF2-001 - ENSP00000356506 [100%] 
NCF2-002 - ENSP00000356505 [100%] 
NCF2-201 - ENSP00000399294 [100%] 
NCF2-202 - ENSP00000407217 [100%] 

											 
																						NCF2-001 - ENSP00000356506 [99%] 
NCF2-002 - ENSP00000356505 [99%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NCF2-001
NCF2-002
NCF2-201
NCF2-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002327

												
										 
																				
											
											
											
Antibody HPA006040

												
										 
																				
											
											
											
Antibody CAB022160

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Differential expression of ANXA6, HSP27, PRDX2, NCF2, and TPM4 during uterine cervix carcinogenesis: diagnostic and prognostic value

	Lomnytska MI et al

	Br J Cancer 2011;104(1):110-9

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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