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Antibody staining overlaps with antibody HPA027141.
Validated
Antibody staining overlaps with antibody HPA027104.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Antibody dilution
1:400
1:20
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027104
Antibody HPA027141
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human thyroid gland shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine cells.
Expression
RNA: detected in 37 tissues Protein: detected in 79 cell types
RNA: detected in 37 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:900
1:60
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027104
Antibody HPA027141
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402619)
Target mass (kDa)
38.9
38.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA027104
Antibody HPA027141
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
