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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & plasma membrane.
Antibody dilution
1:50
1:200
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA028436
Antibody HPA052655
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
Antibody dilution
1:80
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028436
Antibody HPA052655
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408064)
Target mass (kDa)
44.6, 43.3, 26.4
44.6, 43.3, 38.2
Antibody dilution
1:250
1:1330
PROTEIN ARRAY
Antibody HPA028436
Antibody HPA052655
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
