Fields »
ABOUT
HELP
BLOG
MRPS15

ANTIBODY INFORMATION
 
 

Antibody HPA028100

  

Antibody HPA028134

  

Antibody HPA067137

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA028100   HPA028134   HPA067137  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 6   6   16  

References

   1    

Proper citation

 Atlas Antibodies Cat#HPA028100, RRID:AB_10600216   Atlas Antibodies Cat#HPA028134, RRID:AB_10600890   Atlas Antibodies Cat#HPA067137, RRID:AB_2685787  

VALIDATION SUMMARY
 
 

Antibody HPA028100

  

Antibody HPA028134

  

Antibody HPA067137

  
Immunocytochemistry

Uncertain     Supported  
Immunohistochemistry

Supported   Supported    
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028100

  

Antibody HPA028134

  

Antibody HPA067137

  

Standard validation

 Uncertain     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & mitochondria.     Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:50     1:74  

Literature conformity

 The subcellular location is not consistent with literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028100

  

Antibody HPA028134

  

Antibody HPA067137

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:20   1:15    

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA028100

  

Antibody HPA028134

  

Antibody HPA067137

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 29.8   29.8   29.8  

Antibody dilution

 1:250   1:250   1:240  

PROTEIN ARRAY
 
 

Antibody HPA028100

  

Antibody HPA028134

  

Antibody HPA067137

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500   1:2950  

ANTIGEN INFORMATION
 
 

Antibody HPA028100

  

Antibody HPA028134

  

Antibody HPA067137

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 73   72   71  

Antigen sequence

 
GLGIEYTFPPLYYRRAHRRFVTKKALCIRVFQETQKLKKRRRALKAAAAA
QKQAKRRNPDSPAKAIPKTLKDS
  
AKFPFNQWGLQPRSLLLQAARGYVVRKPAQSRLDDDPPPSTLLKDYQNVP
GIEKVDDVVKRLLSLEMANKKE
  
EQFMKKIVANPEDTRSLEARIIALSVKIRSYEEHLEKHRKDKAHKRYLLM
SIDQRKKMLKNLRNTNYDVFE
 

Matching transcripts

 MRPS15-001 - ENSP00000362208 [100%]
  MRPS15-001 - ENSP00000362208 [100%]
  MRPS15-001 - ENSP00000362208 [100%]
 

Other gene match

      
ANTIGEN VIEW
MRPS15-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA028100

  

Antibody HPA028134

  

Antibody HPA067137

  

1.

   Mitochondria "fuel" breast cancer metabolism: fifteen markers of mitochondrial biogenesis label epithelial cancer cells, but are excluded from adjacent stromal cells
Sotgia F et al
Cell Cycle 2012;11(23):4390-401
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.