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Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.
Antibody dilution
1:4
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA012734
Antibody HPA030491
Standard validation
Approved
Figure description
Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in chief cells.
Expression
RNA: detected in 37 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
Antibody dilution
1:15
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA012734
Antibody HPA030491
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404450)
Target mass (kDa)
40.4, 35.8, 34, 32.6
40.4, 35.8, 32.6, 23.2, 18.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA012734
Antibody HPA030491
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
