We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Fields »
 

Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
ABOUT
HELP
BLOG
  • THE HUMAN PROTEOME
  • THE HUMAN TISSUES
  • THE HUMAN CELL
  • HUMAN PATHOLOGY
  • PROTEIN CLASSES
  • PROTEIN EVIDENCE
    • NEWS
    • BLOG
    • EVENTS
    • PRESS ROOM
    • LEARN
    • DICTIONARY
    • METHODS
    • CELL LINES
  • THE PROJECT
  • INTRODUCTION
  • ORGANIZATION
  • PUBLICATIONS
  • PUBLICATION DATA
  • ANTIBODY SUBMISSION
  • ANTIBODY AVAILABILITY
  • LINKS
  • CONTACT
  • TECHNICAL DATA
  • ANTIBODY VALIDATION
  • ASSAYS & ANNOTATION
  • DISCLAIMER
  • DOWNLOADABLE DATA
  • HELP & FAQ
  • LICENCE & CITATION
  • PRIVACY STATEMENT
  • RELEASE HISTORY
ATP6V0B
  • TISSUE

  • CELL

  • PATHOLOGY

ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA044281

 

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA044281  

Host species

Rabbit  

Clonality

pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 

Released in version

10  

References

 

Proper citation

 

VALIDATION SUMMARY

 
 

Antibody HPA044281

 
Immunocytochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA044281

 

Standard validation

Uncertain  

 

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

1:77  

Literature conformity

The subcellular location is not consistent with literature.  

WESTERN BLOT

 
 

Antibody HPA044281

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
 

 

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

27.7, 26.1, 21.4, 16.2  

Antibody dilution

1:250  

PROTEIN ARRAY

 
 

Antibody HPA044281

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
 

 

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:500  

ANTIGEN INFORMATION

 
 

Antibody HPA044281

 

Antigen

Recombinant protein fragment  

Length (aa)

26  

Antigen sequence

EPFSATDPKAIGHRNYHAGYSMFGAG
 

Matching transcripts

ATP6V0B-001 - ENSP00000431605 [100%]
ATP6V0B-004 - ENSP00000236067 [100%]
ATP6V0B-006 - ENSP00000432754 [100%]
ATP6V0B-011 - ENSP00000434094 [100%]
ATP6V0B-012 - ENSP00000434729 [100%]
 

Other gene match

 
ANTIGEN VIEW
ATP6V0B-001
ATP6V0B-004
ATP6V0B-006
ATP6V0B-011
ATP6V0B-012
 

Contact

  • BLOG
  • PRESS ROOM
  • contact@proteinatlas.org

The Project

  • INTRODUCTION
  • ORGANIZATION
  • PUBLICATIONS
  • DOWNLOADABLE DATA

The Human Protein Atlas

  • ANTIBODY AVAILABILITY
  • LICENCE & CITATION
  • HELP & FAQ
The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.