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TTR

ANTIBODY INFORMATION
 
 

Antibody HPA002550

  

Antibody CAB002517

  

Antibody CAB062567

  

Antibody CAB073406

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Novocastra
   Atlas Antibodies
Sigma-Aldrich
   NCI-CPTC
  

Product name

 HPA002550   NCL-PREp   AMAb90649   CPTC-TTR-1  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 3   1   12   13  

References

 4        

Proper citation

 Atlas Antibodies Cat#HPA002550, RRID:AB_1080419     Atlas Antibodies Cat#AMAb90649, RRID:AB_2665620    

VALIDATION SUMMARY
 
 

Antibody HPA002550

  

Antibody CAB002517

  

Antibody CAB062567

  

Antibody CAB073406

  
Immunocytochemistry

Approved        
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Approved        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002550

  

Antibody CAB002517

  

Antibody CAB062567

  

Antibody CAB073406

  

Standard validation

 Approved        

       

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to the Golgi apparatus.        

Antibody dilution

 1:57        

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002550

  

Antibody CAB002517

  

Antibody CAB062567

  

Antibody CAB073406

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human pancreas shows selective positivity in islets of Langerhans.   Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human pancreas shows selective positivity in islets of Langerhans.   Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 22 tissues
Protein: detected in 4 cell types		   RNA: detected in 22 tissues
Protein: detected in 31 cell types		   RNA: detected in 22 tissues
Protein: detected in 4 cell types		   RNA: detected in 22 tissues
Protein: detected in 6 cell types		  

Retrieval

 HIER pH6   None   HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:600   1:8000   1:8000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002550

  

Antibody CAB002517

  

Antibody CAB062567

  

Antibody CAB073406

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 15.9, 15.1   20.1, 15.9, 15.1   20.1, 15.9, 15.1   20.1, 15.9, 15.1  

Antibody dilution

 1:250   1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002550

  

Antibody CAB002517

  

Antibody CAB062567

  

Antibody CAB073406

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		        

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000        

ANTIGEN INFORMATION
 
 

Antibody HPA002550

  

Antibody CAB002517

  

Antibody CAB062567

  

Antibody CAB073406

  

Antigen

 Recombinant protein fragment   Not known   Recombinant protein   Recombinant protein  

Length (aa)

 124        

Antigen sequence

 
GTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESG
ELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPR
RYTIAALLSPYSYSTTAVVTNPKE
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					TTR-001 - ENSP00000237014 [100%] 
TTR-202 - ENSP00000479174 [94%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TTR-001
TTR-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002550

												
										 
																				
											
											
											
Antibody CAB002517

												
										 
																				
											
											
											
Antibody CAB062567

												
										 
																				
											
											
											
Antibody CAB073406

												
										 
																		

																	

										
											
1.

												
																						Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Heparan sulfate/heparin promotes transthyretin fibrillization through selective binding to a basic motif in the protein

	Noborn F et al

	Proc Natl Acad Sci U S A 2011;108(14):5584-9

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Ophthalmic manifestations in a Chinese family with familial amyloid polyneuropathy due to a TTR Gly83Arg mutation

	Liu T et al

	Eye (Lond) 2014;28(1):26-33

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Substoichiometric inhibition of transthyretin misfolding by immune-targeting sparsely populated misfolding intermediates: a potential diagnostic and therapeutic for TTR amyloidoses

	Galant NJ et al

	Sci Rep 2016;6:25080

			Application: WB				
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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