Expression of SPP1 in cancer - Summary - The Human Protein Atlas

GENERAL INFORMATION
Gene name	SPP1
Gene description	Secreted phosphoprotein 1
Protein class	Cancer-related genes Plasma proteins Predicted secreted proteins
Predicted localization	Secreted
Number of transcripts	5
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Group enriched (EFO-21, Hep G2, RPTEC TERT1)
HPA (normal tissue):	Tissue enhanced (gallbladder, kidney, placenta)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in a subset of tissues, most abundant in renal tubules and gallbladder.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data. Secreted protein, tissue location of RNA and protein might differ and correlation is complex.
Reliability score	Approved based on 2 antibodies. HPA027541 , CAB002212
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PROGNOSTIC SUMMARY
Prognostic marker in liver cancer (unfavourable), pancreatic cancer (unfavourable) and cervical cancer (unfavourable).
Liver cancer p<0.001 Pancreatic cancer p<0.001 Cervical cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

HPA027541	CAB002212

GENE INFORMATION
Gene name	SPP1 (HGNC Symbol)
Synonyms	BNSP, BSPI, ETA-1, OPN
Description	Secreted phosphoprotein 1 (HGNC Symbol)
Entrez gene summary	The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Chromosome	4
Cytoband	q22.1
Chromosome location (bp)	87975650 - 87983426
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000118785 (version 83.38)
Entrez gene	6696
UniProt	P10451 (UniProt - Evidence at protein level)
neXtProt	NX_P10451
Antibodypedia	SPP1 antibodies

PROTEIN BROWSER

SPP1-001	SPP1-002	SPP1-003	SPP1-010	SPP1-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
SPP1-001 ENSP00000378517 ENST00000395080	P10451 [Direct mapping] Osteopontin A0A024RDE2 [Target identity:100%; Query identity:100%] Secreted phosphoprotein 1 (Osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), isoform CRA_b Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001503 [ossification] GO:0001649 [osteoblast differentiation] GO:0005125 [cytokine activity] GO:0005515 [protein binding] GO:0005576 [extracellular region] GO:0005615 [extracellular space] GO:0005737 [cytoplasm] GO:0006954 [inflammatory response] GO:0007155 [cell adhesion] GO:0007566 [embryo implantation] GO:0010033 [response to organic substance] GO:0010811 [positive regulation of cell-substrate adhesion] GO:0022617 [extracellular matrix disassembly] GO:0030154 [cell differentiation] GO:0030198 [extracellular matrix organization] GO:0030593 [neutrophil chemotaxis] GO:0031214 [biomineral tissue development] GO:0031988 [membrane-bounded vesicle] GO:0033280 [response to vitamin D] GO:0042995 [cell projection] GO:0045177 [apical part of cell] GO:0045780 [positive regulation of bone resorption] GO:0046697 [decidualization] GO:0048471 [perinuclear region of cytoplasm] GO:0048545 [response to steroid hormone] GO:0048685 [negative regulation of collateral sprouting of intact axon in response to injury] GO:0050840 [extracellular matrix binding] GO:0070062 [extracellular exosome] GO:0071498 [cellular response to fluid shear stress] Show all	314 aa 35.4 kDa	Yes	0
SPP1-002 ENSP00000237623 ENST00000237623	P10451 [Direct mapping] Osteopontin A0A024RDE6 [Target identity:100%; Query identity:100%] Secreted phosphoprotein 1 (Osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), isoform CRA_c Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001503 [ossification] GO:0005125 [cytokine activity] GO:0005515 [protein binding] GO:0005576 [extracellular region] GO:0005615 [extracellular space] GO:0007155 [cell adhesion] GO:0007566 [embryo implantation] GO:0022617 [extracellular matrix disassembly] GO:0030198 [extracellular matrix organization] GO:0031214 [biomineral tissue development] GO:0033280 [response to vitamin D] GO:0046697 [decidualization] GO:0070062 [extracellular exosome] Show all	300 aa 33.8 kDa	Yes	0
SPP1-003 ENSP00000354042 ENST00000360804	P10451 [Direct mapping] Osteopontin A0A024RDJ0 [Target identity:100%; Query identity:100%] Secreted phosphoprotein 1 (Osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), isoform CRA_a Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001503 [ossification] GO:0005125 [cytokine activity] GO:0005515 [protein binding] GO:0005576 [extracellular region] GO:0005615 [extracellular space] GO:0007155 [cell adhesion] GO:0007566 [embryo implantation] GO:0022617 [extracellular matrix disassembly] GO:0030198 [extracellular matrix organization] GO:0031214 [biomineral tissue development] GO:0033280 [response to vitamin D] GO:0046697 [decidualization] GO:0070062 [extracellular exosome] Show all	287 aa 32.4 kDa	Yes	0
SPP1-010 ENSP00000422973 ENST00000508233	D6R9C5 [Direct mapping] Osteopontin Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0001503 [ossification] GO:0007155 [cell adhesion] Show all	241 aa 27.1 kDa	Yes	0
SPP1-201 ENSP00000477824 ENST00000614857	P10451 [Direct mapping] Osteopontin F2YQ21 [Target identity:100%; Query identity:100%] Osteopontin-D Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Ezkurdia et al 2014) Show all	GO:0001503 [ossification] GO:0005125 [cytokine activity] GO:0005515 [protein binding] GO:0005576 [extracellular region] GO:0005615 [extracellular space] GO:0007155 [cell adhesion] GO:0007566 [embryo implantation] GO:0022617 [extracellular matrix disassembly] GO:0030198 [extracellular matrix organization] GO:0031214 [biomineral tissue development] GO:0033280 [response to vitamin D] GO:0046697 [decidualization] GO:0070062 [extracellular exosome] Show all	292 aa 33 kDa	Yes	0