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CCND2

ANTIBODY INFORMATION
 
 

Antibody HPA049138

  

Antibody HPA054196

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049138   HPA054196  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   13  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA049138, RRID:AB_2680650   Atlas Antibodies Cat#HPA054196, RRID:AB_2682412  

VALIDATION SUMMARY
 
 

Antibody HPA049138

  

Antibody HPA054196

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049138

  

Antibody HPA054196

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA054196.		  
Validated

Antibody staining overlaps with antibody HPA049138.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.  

Antibody dilution

 1:200   1:80  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049138

  

Antibody HPA054196

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human epididymis shows nuclear positivity in glandular cells.    

Expression

 RNA: detected in 36 tissues
Protein: detected in 32 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:250    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA049138

  

Antibody HPA054196

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 33.1   33.1  

Antibody dilution

 1:1150   1:400  

PROTEIN ARRAY
 
 

Antibody HPA049138

  

Antibody HPA054196

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:9200   1:3200  

ANTIGEN INFORMATION
 
 

Antibody HPA049138

  

Antibody HPA054196

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 43   48  

Antigen sequence

 
QEQIEAVLLNSLQQYRQDQRDGSKSEDELDQASTPTDVRGIDL
  
VRDRNLLRDDRVLQNLLTIEERYLPQCSYFKCVQKDIQPYMRRMVATW
 

Matching transcripts

 CCND2-001 - ENSP00000261254 [98%]
  CCND2-001 - ENSP00000261254 [100%]
 

Other gene match

    
ANTIGEN VIEW
CCND2-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA049138

  

Antibody HPA054196

  

1.

 RNA deep sequencing as a tool for selection of cell lines for systematic subcellular localization of all human proteins
Danielsson F et al
J Proteome Res 2013;12(1):299-307
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.