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ANTIBODY INFORMATION
 
 

Antibody HPA042889

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042889  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 8  

References

  

Proper citation

 Atlas Antibodies Cat#HPA042889, RRID:AB_10795515  

VALIDATION SUMMARY
 
 

Antibody HPA042889

  
Immunocytochemistry

Uncertain  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042889

  

Standard validation

 Uncertain  

 

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:5  

Literature conformity

 The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042889

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human uterus, pre-menopause shows moderate cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 29 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:15  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042889

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 44.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA042889

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA042889

  

Antigen

 Recombinant protein fragment  

Length (aa)

 109  

Antigen sequence

 
NSCAYKVGVASGHLPRKGSGSDCRLGHNAFSWVFSRYDQEFRFSHNGQHE
PLGLLRGPAQLGVVLDLQVQELLFYEPASGTVLCAHHVSFPGPLFPVFAV
ADQTISIVR
 

Matching transcripts

 BSPRY-001 - ENSP00000363298 [100%]
 

Other gene match

  
ANTIGEN VIEW
BSPRY-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.