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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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DNMT3A
  • TISSUE

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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

DNMT3A

Gene description

DNA (cytosine-5-)-methyltransferase 3 alpha

Protein class

Cancer-related genes
Disease related genes
Enzymes
Plasma proteins
Potential drug targets
Predicted intracellular proteins
Transcription factors

Predicted localization

Intracellular

Number of transcripts

5
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (SH-SY5Y)
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Ubiquitous nuclear expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Approved based on 2 antibodies.
HPA026588 , CAB009469
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in pancreatic cancer (favourable), endometrial cancer (unfavourable), liver cancer (unfavourable) and renal cancer (unfavourable).
Pancreatic cancer p<0.001
Endometrial cancer p<0.001
Liver cancer p<0.001
Renal cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA026588
CAB009469

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues showed moderate to strong nuclear staining and a few with additional cytoplasmic positivity.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer cells showed nuclear and cytoplasmic staining of varying intensity. Lymphomas were mainly negative.

GENE INFORMATION

Gene name

DNMT3A

Synonyms

Description

DNA (cytosine-5-)-methyltransferase 3 alpha (HGNC Symbol)

Entrez gene summary

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Chromosome

2

Cytoband

p23.3

Chromosome location (bp)

25227855 - 25342590

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000119772 (version 83.38)

Entrez gene

1788

UniProt

Q9Y6K1 (UniProt - Evidence at protein level)

neXtProt

NX_Q9Y6K1

Antibodypedia

DNMT3A antibodies

PROTEIN BROWSER

DNMT3A-001
DNMT3A-002
DNMT3A-011
DNMT3A-012
DNMT3A-013

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

DNMT3A-001
ENSP00000264709
ENST00000264709
Q9Y6K1 [Direct mapping]
DNA (cytosine-5)-methyltransferase 3A
Show all
Enzymes
   ENZYME proteins
   Transferases
Predicted intracellular proteins
Plasma proteins
Transcription factors
   Zinc-coordinating DNA-binding domains
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Frameshift Mutations
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000122 [negative regulation of transcription from RNA polymerase II promoter]
GO:0000278 [mitotic cell cycle]
GO:0000775 [chromosome, centromeric region]
GO:0000791 [euchromatin]
GO:0000792 [heterochromatin]
GO:0001741 [XY body]
GO:0003677 [DNA binding]
GO:0003682 [chromatin binding]
GO:0003886 [DNA (cytosine-5-)-methyltransferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005720 [nuclear heterochromatin]
GO:0005737 [cytoplasm]
GO:0006306 [DNA methylation]
GO:0006325 [chromatin organization]
GO:0006346 [methylation-dependent chromatin silencing]
GO:0006349 [regulation of gene expression by genetic imprinting]
GO:0007283 [spermatogenesis]
GO:0007568 [aging]
GO:0008168 [methyltransferase activity]
GO:0009008 [DNA-methyltransferase activity]
GO:0009636 [response to toxic substance]
GO:0010212 [response to ionizing radiation]
GO:0010288 [response to lead ion]
GO:0010424 [DNA methylation on cytosine within a CG sequence]
GO:0010467 [gene expression]
GO:0010942 [positive regulation of cell death]
GO:0016363 [nuclear matrix]
GO:0030182 [neuron differentiation]
GO:0031667 [response to nutrient levels]
GO:0032355 [response to estradiol]
GO:0032776 [DNA methylation on cytosine]
GO:0033189 [response to vitamin A]
GO:0036276 [response to antidepressant]
GO:0040029 [regulation of gene expression, epigenetic]
GO:0042220 [response to cocaine]
GO:0042493 [response to drug]
GO:0042802 [identical protein binding]
GO:0043045 [DNA methylation involved in embryo development]
GO:0043046 [DNA methylation involved in gamete generation]
GO:0044027 [hypermethylation of CpG island]
GO:0045322 [unmethylated CpG binding]
GO:0045471 [response to ethanol]
GO:0045814 [negative regulation of gene expression, epigenetic]
GO:0046498 [S-adenosylhomocysteine metabolic process]
GO:0046499 [S-adenosylmethioninamine metabolic process]
GO:0046872 [metal ion binding]
GO:0051718 [DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates]
GO:0071230 [cellular response to amino acid stimulus]
GO:0071361 [cellular response to ethanol]
GO:0071456 [cellular response to hypoxia]
GO:0090116 [C-5 methylation of cytosine]
GO:0097284 [hepatocyte apoptotic process]
Show all
912 aa
101.9 kDa
No 0
DNMT3A-002
ENSP00000370122
ENST00000380746
Q9Y6K1 [Direct mapping]
DNA (cytosine-5)-methyltransferase 3A
Show all
Enzymes
   ENZYME proteins
   Transferases
Predicted intracellular proteins
Plasma proteins
Transcription factors
   Zinc-coordinating DNA-binding domains
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Frameshift Mutations
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000791 [euchromatin]
GO:0003677 [DNA binding]
GO:0003886 [DNA (cytosine-5-)-methyltransferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0006306 [DNA methylation]
GO:0006325 [chromatin organization]
GO:0006349 [regulation of gene expression by genetic imprinting]
GO:0008168 [methyltransferase activity]
GO:0009008 [DNA-methyltransferase activity]
GO:0010467 [gene expression]
GO:0016363 [nuclear matrix]
GO:0032776 [DNA methylation on cytosine]
GO:0040029 [regulation of gene expression, epigenetic]
GO:0042802 [identical protein binding]
GO:0045814 [negative regulation of gene expression, epigenetic]
GO:0046872 [metal ion binding]
GO:0090116 [C-5 methylation of cytosine]
Show all
723 aa
81.6 kDa
No 0
DNMT3A-011
ENSP00000324375
ENST00000321117
Q9Y6K1 [Direct mapping]
DNA (cytosine-5)-methyltransferase 3A
Show all
Enzymes
   ENZYME proteins
   Transferases
Predicted intracellular proteins
Plasma proteins
Transcription factors
   Zinc-coordinating DNA-binding domains
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Frameshift Mutations
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000791 [euchromatin]
GO:0003677 [DNA binding]
GO:0003886 [DNA (cytosine-5-)-methyltransferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0006306 [DNA methylation]
GO:0006325 [chromatin organization]
GO:0006349 [regulation of gene expression by genetic imprinting]
GO:0008168 [methyltransferase activity]
GO:0009008 [DNA-methyltransferase activity]
GO:0010467 [gene expression]
GO:0016363 [nuclear matrix]
GO:0032776 [DNA methylation on cytosine]
GO:0040029 [regulation of gene expression, epigenetic]
GO:0042802 [identical protein binding]
GO:0045814 [negative regulation of gene expression, epigenetic]
GO:0046872 [metal ion binding]
GO:0090116 [C-5 methylation of cytosine]
Show all
912 aa
101.9 kDa
No 0
DNMT3A-012
ENSP00000384852
ENST00000406659
Q9Y6K1 [Direct mapping]
DNA (cytosine-5)-methyltransferase 3A
Show all
Enzymes
   ENZYME proteins
   Transferases
Predicted intracellular proteins
Plasma proteins
Transcription factors
   Zinc-coordinating DNA-binding domains
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Frameshift Mutations
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000791 [euchromatin]
GO:0003677 [DNA binding]
GO:0003886 [DNA (cytosine-5-)-methyltransferase activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0006306 [DNA methylation]
GO:0006325 [chromatin organization]
GO:0006349 [regulation of gene expression by genetic imprinting]
GO:0009008 [DNA-methyltransferase activity]
GO:0010467 [gene expression]
GO:0016363 [nuclear matrix]
GO:0032776 [DNA methylation on cytosine]
GO:0040029 [regulation of gene expression, epigenetic]
GO:0042802 [identical protein binding]
GO:0045814 [negative regulation of gene expression, epigenetic]
GO:0046872 [metal ion binding]
GO:0090116 [C-5 methylation of cytosine]
Show all
166 aa
16.9 kDa
No 0
DNMT3A-013
ENSP00000384237
ENST00000402667
A0A0C4DG02 [Direct mapping]
DNA (cytosine-5)-methyltransferase 3A
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Nonsense Mutations
   COSMIC Missense Mutations
   COSMIC Frameshift Mutations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0006306 [DNA methylation]
GO:0008168 [methyltransferase activity]
GO:0009008 [DNA-methyltransferase activity]
Show all
689 aa
77.8 kDa
No 0

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The Human Protein Atlas project is funded
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