Fields »
ABOUT
HELP
BLOG
CRIPT

ANTIBODY INFORMATION
 
 

Antibody HPA046080

  

Antibody HPA056765

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046080   HPA056765  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA046080, RRID:AB_2679529   Atlas Antibodies Cat#HPA056765, RRID:AB_2683230  

VALIDATION SUMMARY
 
 

Antibody HPA046080

  

Antibody HPA056765

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046080

  

Antibody HPA056765

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA056765.		  
Validated

Antibody staining overlaps with antibody HPA046080.		  
 

Standard validation

 Approved   Supported  

   

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus & nucleoli fibrillar center.   Immunofluorescent staining of human cell line A-431 shows localization to nucleus.  

Antibody dilution

 1:40   1:41  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046080

  

Antibody HPA056765

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatoctytes.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 21 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:250    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA046080

  

Antibody HPA056765

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 11.2   11.2  

Antibody dilution

 1:200   1:140  

PROTEIN ARRAY
 
 

Antibody HPA046080

  

Antibody HPA056765

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1600   1:1650  

ANTIGEN INFORMATION
 
 

Antibody HPA046080

  

Antibody HPA056765

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   26  

Antigen sequence

 
CEKKLGTVITPDTWKDGARNTTESGGRKLNENKALTSKKARFDPYGKNKF
STCRICKSSVHQPGSHYCQGC
  
CAYKKGICAMCGKKVLDTKNYKQTSV
 

Matching transcripts

 CRIPT-001 - ENSP00000238892 [100%]
  CRIPT-001 - ENSP00000238892 [100%]
 

Other gene match

    
ANTIGEN VIEW
CRIPT-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.