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PYROXD2

ANTIBODY INFORMATION
 
 

Antibody HPA037566

  

Antibody HPA037567

  

Antibody HPA067394

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037566   HPA037567   HPA067394  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 7   12   14  

References

      

Proper citation

 Atlas Antibodies Cat#HPA037566, RRID:AB_10670385   Atlas Antibodies Cat#HPA037567, RRID:AB_2675550   Atlas Antibodies Cat#HPA067394, RRID:AB_2685832  

VALIDATION SUMMARY
 
 

Antibody HPA037566

  

Antibody HPA037567

  

Antibody HPA067394

  
Immunocytochemistry

    Approved  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037566

  

Antibody HPA037567

  

Antibody HPA067394

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

     1:200  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037566

  

Antibody HPA037567

  

Antibody HPA067394

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.    

Expression

 RNA: detected in 36 tissues
Protein: detected in 61 cell types		   RNA: detected in 36 tissues
Protein: detected in 44 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:100   1:60    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA037566

  

Antibody HPA037567

  

Antibody HPA067394

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 63.1   63.1   63.1  

Antibody dilution

 1:250   1:250   1:1190  

PROTEIN ARRAY
 
 

Antibody HPA037566

  

Antibody HPA037567

  

Antibody HPA067394

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:14300  

ANTIGEN INFORMATION
 
 

Antibody HPA037566

  

Antibody HPA037567

  

Antibody HPA067394

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   98   87  

Antigen sequence

 
VVSLFTQYTPYTLAGGKAWDEQERDAYADRVFDCIEVYAPGFKDSVVGRD
ILTPPDLERIFGLPGGNIFHCAMSLDQLYFTRPVPLHSGYRC
  
RLHLRNPYSFTPMLEEGAGSKVPRCLLLGTDMAENQKQIAQFSQKDAQVF
PKYEEFMHRLALAIDPLLDAAPVDMAAFQHGSLLQRMRSLSTLKPLLK
  
VLTAPITKVLDQWFESEPLKATLATDAVIGAMTSPHTPGSGYVLLHHVMG
GLEGMQGAWGYVQGGMGALSDAIASSATTHGASIFTE
 

Matching transcripts

 PYROXD2-001 - ENSP00000359607 [98%]
  PYROXD2-001 - ENSP00000359607 [100%]
  PYROXD2-001 - ENSP00000359607 [100%]
 

Other gene match

      
ANTIGEN VIEW
PYROXD2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.