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ADRA1A

ANTIBODY INFORMATION
 
 

Antibody HPA029678

  

Antibody HPA029679

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA029678   HPA029679  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA029678, RRID:AB_10602108   Atlas Antibodies Cat#HPA029679, RRID:AB_2673143  

VALIDATION SUMMARY
 
 

Antibody HPA029678

  

Antibody HPA029679

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029678

  

Antibody HPA029679

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in subsets of myocytes.   Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in astrocytes.  

Expression

 RNA: detected in 16 tissues
Protein: detected in 24 cell types		   RNA: detected in 16 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:65   1:8  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA029678

  

Antibody HPA029679

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 52.2, 51.5, 50.8, 47.5, 47.2   51.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA029678

  

Antibody HPA029679

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA029678

  

Antibody HPA029679

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 65   58  

Antigen sequence

 
KAFQNVLRIQCLRRKQSSKHALGYTLHPPSQAVEGQHKDMVRIPVGSRET
FYRISKTDGVCEWKF
  
SARITVSKDQSSCTTARVRSKSFLQVCCCVGPSTPSLDKNHQVPTIKVHT
ISLSENGE
 

Matching transcripts

 ADRA1A-001 - ENSP00000369956 [98%]
ADRA1A-002 - ENSP00000346557 [98%]
ADRA1A-003 - ENSP00000276393 [98%]
ADRA1A-004 - ENSP00000369960 [98%]
ADRA1A-008 - ENSP00000369947 [98%]
ADRA1A-009 - ENSP00000430793 [98%]
  ADRA1A-003 - ENSP00000276393 [100%]
ADRA1A-008 - ENSP00000369947 [100%]
 

Other gene match

    
ANTIGEN VIEW
ADRA1A-001
ADRA1A-002
ADRA1A-003
ADRA1A-004
ADRA1A-008
ADRA1A-009
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.